diff --git a/graphics/openslide/Makefile b/graphics/openslide/Makefile
index 8ce31d1bc1a2..283f936d8ed3 100644
--- a/graphics/openslide/Makefile
+++ b/graphics/openslide/Makefile
@@ -1,27 +1,28 @@
 PORTNAME=	openslide
 DISTVERSIONPREFIX=	v
-DISTVERSION=	4.0.0
-PORTREVISION=	3
+DISTVERSION=	4.0.1
 CATEGORIES=	graphics
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	C library for reading virtual slide images
 WWW=		https://openslide.org \
 		https://github.com/openslide/openslide
 
 LICENSE=	LGPL21
 LICENSE_FILE=	${WRKSRC}/COPYING.LESSER
 
+BUILD_DEPENDS=	xdelta3:misc/xdelta3
 LIB_DEPENDS=	libdicom.so:graphics/libdicom \
 		libopenjp2.so:graphics/openjpeg \
 		libpng16.so:graphics/png \
 		libtiff.so:graphics/tiff
 
 USES=		gettext-runtime gnome jpeg meson pkgconfig sqlite
 USE_GITHUB=	yes
 USE_GNOME=	cairo gdkpixbuf glib20 libxml2
 USE_LDCONFIG=	yes
 
-MESON_ARGS=	-Ddoc=disabled
+MESON_ARGS=	-Ddoc=disabled \
+		-Dtest=disabled
 
 .include <bsd.port.mk>
diff --git a/graphics/openslide/distinfo b/graphics/openslide/distinfo
index 5f89b8b363b1..18d9e17a866c 100644
--- a/graphics/openslide/distinfo
+++ b/graphics/openslide/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1703143508
-SHA256 (openslide-openslide-v4.0.0_GH0.tar.gz) = 0845f3bc5db660ccff890602efc0167524f6269dd20c49a9a5b469658dbb603c
-SIZE (openslide-openslide-v4.0.0_GH0.tar.gz) = 217159
+TIMESTAMP = 1780899354
+SHA256 (openslide-openslide-v4.0.1_GH0.tar.gz) = 25512985b0a539d83f76f0f63aa57ef4430b0ca04a06ae63281d9eadd414f02f
+SIZE (openslide-openslide-v4.0.1_GH0.tar.gz) = 247000
diff --git a/graphics/openslide/pkg-plist b/graphics/openslide/pkg-plist
index 874baeef8b3c..e9b76aca2ebf 100644
--- a/graphics/openslide/pkg-plist
+++ b/graphics/openslide/pkg-plist
@@ -1,14 +1,14 @@
 bin/openslide-quickhash1sum
 bin/openslide-show-properties
 bin/openslide-write-png
 bin/slidetool
 include/openslide/openslide-features.h
 include/openslide/openslide.h
 lib/libopenslide.so
 lib/libopenslide.so.1
-lib/libopenslide.so.1.0.0
+lib/libopenslide.so.1.0.1
 libdata/pkgconfig/openslide.pc
 share/man/man1/openslide-quickhash1sum.1.gz
 share/man/man1/openslide-show-properties.1.gz
 share/man/man1/openslide-write-png.1.gz
 share/man/man1/slidetool.1.gz