diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index a58c6f20115a..2ddb5ee645d8 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,32 +1,31 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.10.0
-PORTREVISION=	9
+DISTVERSION=	2.10.1
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 WWW=		https://bioinf.shenwei.me/seqkit \
 		https://github.com/shenwei356/seqkit
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 USE_GITHUB=	nodefault
 GH_TUPLE=	golang:sys:v0.6.0:sys
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 pre-patch:
 	${RM} -r ${WRKSRC}/vendor/golang.org/x/sys
 	${LN} -s ${WRKDIR}/sys-* ${WRKSRC}/vendor/golang.org/x/sys
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 15f7c568f012..04dc98eed337 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,7 +1,7 @@
-TIMESTAMP = 1741844878
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.mod) = 1c6d38c7d0f78167b5b058b257703084f184e420c2aa7750e0881e4e974ade28
-SIZE (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.mod) = 2514
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.zip) = ca5ef994e7f02ed4e40efefb3dd32833c335c8920fd369af3e44167421609b05
-SIZE (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.zip) = 34094634
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
-SIZE (go/biology_seqkit/seqkit-v2.10.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234
+TIMESTAMP = 1762963353
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.mod) = e2c10d5a885a4ab21cc40f5665d8501580c40e8f136add7ee8b6d6f1e9395ca2
+SIZE (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.mod) = 2516
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.zip) = 22d1ed2e8ac5a878948b832f52519cfa3e51f4b23ccec0ccbdc97e5e71a9ae81
+SIZE (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.zip) = 34096290
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
+SIZE (go/biology_seqkit/seqkit-v2.10.1/golang-sys-v0.6.0_GH0.tar.gz) = 1434234