diff --git a/biology/kallisto/Makefile b/biology/kallisto/Makefile
index 42715d9abc88..ea7cb1a53596 100644
--- a/biology/kallisto/Makefile
+++ b/biology/kallisto/Makefile
@@ -1,44 +1,48 @@
 PORTNAME=		kallisto
 DISTVERSIONPREFIX=	v
 DISTVERSION=		0.50.0
+PORTREVISION=		1
 PORTEPOCH=		1
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Quantify abundances of transcripts from RNA-Seq data
 WWW=		https://pachterlab.github.io/kallisto/about.html
 
 LICENSE=	BSD2CLAUSE
 LICENSE_FILE=	${WRKSRC}/license.txt
 
+# Requires a 64-bit processor
+ONLY_FOR_ARCHS=	aarch64 amd64 powerpc64 powerpc64le riscv64
+
 LIB_DEPENDS=	libhdf5.so:science/hdf5 \
 		libsz.so:science/libaec \
 		libhts.so:biology/htslib
 
 USES=		cmake:noninja compiler:c++11-lang gmake localbase:ldflags
 USE_GITHUB=	yes
 GH_ACCOUNT=	pachterlab
 
 SUB_FILES=	kallisto-test
 
 PORTEXAMPLES=		*
 PLIST_FILES=		bin/kallisto
 EXAMPLES_PLIST_FILES=	bin/kallisto-test
 
 # hdf5 is being phased out and is no longer built in by default, but is still
 # required for Sleuth (requires kallisto bootstrap estimates) and other
 # downstream tools.  Remove this after Sleuth et al catch up.
 CMAKE_ARGS+=		-DUSE_HDF5:BOOL=ON
 MAKE_JOBS_UNSAFE=	yes
 LDFLAGS+=		-lhts -lz
 
 OPTIONS_DEFINE=	EXAMPLES
 
 post-extract:
 	${RM} -rf ${WRKSRC}/ext/htslib
 
 post-install-EXAMPLES-on:
 	${INSTALL_SCRIPT} ${WRKDIR}/kallisto-test ${STAGEDIR}${PREFIX}/bin
 	cd ${WRKSRC}/test && ${COPYTREE_SHARE} . ${STAGEDIR}${EXAMPLESDIR}
 
 .include <bsd.port.mk>
diff --git a/biology/kallisto/files/patch-ext_bifrost_CMakeLists.txt b/biology/kallisto/files/patch-ext_bifrost_CMakeLists.txt
new file mode 100644
index 000000000000..c4c22a5e0666
--- /dev/null
+++ b/biology/kallisto/files/patch-ext_bifrost_CMakeLists.txt
@@ -0,0 +1,11 @@
+--- ext/bifrost/CMakeLists.txt.orig	2023-07-14 14:27:24 UTC
++++ ext/bifrost/CMakeLists.txt
+@@ -30,8 +30,6 @@ if(COMPILATION_ARCH MATCHES "OFF")
+ 	message("Disabling native architecture compilation (including AVX2)")
+ else(COMPILATION_ARCH MATCHES "OFF")
+ 	message("Compilation architecture: ${COMPILATION_ARCH}")
+-	set(CMAKE_C_FLAGS "${CMAKE_C_FLAGS} -march=${COMPILATION_ARCH}")
+-	set(CMAKE_CXX_FLAGS "${CMAKE_CXX_FLAGS} -march=${COMPILATION_ARCH}")
+ endif(COMPILATION_ARCH MATCHES "OFF")
+ 
+ if(ENABLE_AVX2 MATCHES "OFF")