diff --git a/biology/htslib/Makefile b/biology/htslib/Makefile
index e6daa6552449..383f90e08114 100644
--- a/biology/htslib/Makefile
+++ b/biology/htslib/Makefile
@@ -1,38 +1,38 @@
 PORTNAME=	htslib
-DISTVERSION=	1.15
+DISTVERSION=	1.16
 CATEGORIES=	biology devel
 # Github generated distfiles are incomplete, so use custom tarball
 MASTER_SITES=	https://github.com/samtools/htslib/releases/download/${DISTVERSION}/
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	C library for high-throughput sequencing data formats
 WWW=		https://www.htslib.org/
 
 LICENSE=	BSD3CLAUSE MIT
 LICENSE_COMB=	multi
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 LIB_DEPENDS=	libdeflate.so:archivers/libdeflate
 TEST_DEPENDS=	bash:shells/bash
 
 USES=		autoreconf cpe gmake localbase pathfix perl5 shebangfix tar:bzip2
 USE_PERL5=	test
 USE_LDCONFIG=	yes
 
 GNU_CONFIGURE=		yes
 PATHFIX_MAKEFILEIN=	Makefile
 SHEBANG_FILES=		test/*.pl
 
 OPTIONS_DEFINE=		CURL
 OPTIONS_DEFAULT=	CURL
 
 CURL_DESC=		Use libcurl for network file access (HTTPS and S3 support).
 CURL_USES=		ssl
 CURL_LIB_DEPENDS=	libcurl.so:ftp/curl
 CURL_CONFIGURE_ENABLE=	libcurl
 
 PLIST_SUB=	PORTVERSION="${PORTVERSION}"
 
 TEST_TARGET=	test
 
 .include <bsd.port.mk>
diff --git a/biology/htslib/distinfo b/biology/htslib/distinfo
index 49defada0080..88b848aeb2fb 100644
--- a/biology/htslib/distinfo
+++ b/biology/htslib/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1645831536
-SHA256 (htslib-1.15.tar.bz2) = 1a9f49911503a22f56817cc82ea9b87fb7e7467b5ff989ca5aa61c12e7d532d9
-SIZE (htslib-1.15.tar.bz2) = 4141168
+TIMESTAMP = 1672094487
+SHA256 (htslib-1.16.tar.bz2) = 606b7c7aff73734cf033ecd156f40529fa5792f54524952a28938ca0890d7924
+SIZE (htslib-1.16.tar.bz2) = 4466776