diff --git a/biology/py-pysam/Makefile b/biology/py-pysam/Makefile
index 745a4a165ed1..eedd8dd559bc 100644
--- a/biology/py-pysam/Makefile
+++ b/biology/py-pysam/Makefile
@@ -1,35 +1,37 @@
 PORTNAME=	pysam
 DISTVERSIONPREFIX=	v
-DISTVERSION=	0.22.1
+DISTVERSION=	0.23.0
 CATEGORIES=	biology python
 #MASTER_SITES=	PYPI # no tests
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python module for reading, manipulating and writing genomic data sets
 WWW=		https://github.com/pysam-developers/pysam
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 LIB_DEPENDS=	libhts.so:biology/htslib
 RUN_DEPENDS=	bcftools:biology/bcftools \
 		samtools:biology/samtools
 
 USES=		gmake python
 USE_PYTHON=	distutils autoplist cython cython_run pytest # 2 failed, 155 errors, see https://github.com/pysam-developers/pysam/issues/1284
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	pysam-developers
 
 BINARY_ALIAS=	make=${GMAKE}
 MAKE_ENV=	HTSLIB_MODE=external \
 		HTSLIB_LIBRARY_DIR=${LOCALBASE}/lib \
 		HTSLIB_INCLUDE_DIR=${LOCALBASE}/include
 TEST_ENV=	${MAKE_ENV} PYTHONPATH=${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}
 TEST_WRKSRC=	${WRKSRC}/tests
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pysam/lib*${PYTHON_EXT_SUFFIX}.so
 
+# tests as of 0.23.0: 958 passed, 13 skipped, 1 warning in 502.60s (0:08:22)
+
 .include <bsd.port.mk>
diff --git a/biology/py-pysam/distinfo b/biology/py-pysam/distinfo
index f5f1e4268139..fc2dc1ebfa8a 100644
--- a/biology/py-pysam/distinfo
+++ b/biology/py-pysam/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1714015094
-SHA256 (pysam-developers-pysam-v0.22.1_GH0.tar.gz) = e4981524d7627c53fa0d3f8cbec2bd65c2ea7520092f25e1029af12cb7b82ff6
-SIZE (pysam-developers-pysam-v0.22.1_GH0.tar.gz) = 3885851
+TIMESTAMP = 1738831884
+SHA256 (pysam-developers-pysam-v0.23.0_GH0.tar.gz) = b2a2b66c7e9f6c0b765ba421f46e33d74bfdd797c51ae3020f16c788dd621b0a
+SIZE (pysam-developers-pysam-v0.23.0_GH0.tar.gz) = 4076476