diff --git a/biology/py-crossmap/Makefile b/biology/py-crossmap/Makefile
index 30b8bee8f5af..b36dd2ab99e2 100644
--- a/biology/py-crossmap/Makefile
+++ b/biology/py-crossmap/Makefile
@@ -1,25 +1,24 @@
 PORTNAME=	crossmap
-DISTVERSION=	0.5.2
-PORTREVISION=	1
+DISTVERSION=	0.5.4
 CATEGORIES=	biology python
 MASTER_SITES=	CHEESESHOP
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 DISTNAME=	CrossMap-${DISTVERSION}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Lift over genomics coordinates between assemblies
 
 LICENSE=	GPLv2
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 BUILD_DEPENDS=	${PYTHON_PKGNAMEPREFIX}nose>0.10.4:devel/py-nose@${PY_FLAVOR}
 RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}pysam>0:biology/py-pysam@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}bx-python>0:biology/py-bx-python@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}pyBigWig>0:biology/py-bigwig@${PY_FLAVOR}
 
 USES=		python:3.5+
 USE_PYTHON=	autoplist concurrent cython distutils
 
 NO_ARCH=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/py-crossmap/distinfo b/biology/py-crossmap/distinfo
index e78c67b1ddcf..1dabfc731aef 100644
--- a/biology/py-crossmap/distinfo
+++ b/biology/py-crossmap/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1617575391
-SHA256 (CrossMap-0.5.2.tar.gz) = d996c7a17ca2665903d7bfa4ca67d35c37c800e1ce3129ed1274e65504b93d69
-SIZE (CrossMap-0.5.2.tar.gz) = 10866811
+TIMESTAMP = 1618936542
+SHA256 (CrossMap-0.5.4.tar.gz) = 3419a5bf422754c3acb1e59ec9bba1a6c9a19d3673e962ae832e429ef0f768e5
+SIZE (CrossMap-0.5.4.tar.gz) = 9511082