diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index 1e9d60d295d0..a16c147830e1 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,31 +1,30 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.8.2
-PORTREVISION=	3
+DISTVERSION=	2.9.0
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 WWW=		https://bioinf.shenwei.me/seqkit/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 USE_GITHUB=	nodefault
 GH_TUPLE=	golang:sys:v0.6.0:sys
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 pre-patch:
 	${RM} -r ${WRKSRC}/vendor/golang.org/x/sys
 	${LN} -s ${WRKDIR}/sys-* ${WRKSRC}/vendor/golang.org/x/sys
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 050c34f956e4..ef1e59886d9c 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,7 +1,7 @@
-TIMESTAMP = 1716022666
-SHA256 (go/biology_seqkit/seqkit-v2.8.2/v2.8.2.mod) = 5a4eefdd7e32176c2f257c61a92713f1ed4198aa941ef7a9c2af5d3299b13832
-SIZE (go/biology_seqkit/seqkit-v2.8.2/v2.8.2.mod) = 2514
-SHA256 (go/biology_seqkit/seqkit-v2.8.2/v2.8.2.zip) = ab98d58be18e64aad44928254b939a09e92d7a21ca1d4a31a871ea4aac5e501b
-SIZE (go/biology_seqkit/seqkit-v2.8.2/v2.8.2.zip) = 34091861
-SHA256 (go/biology_seqkit/seqkit-v2.8.2/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
-SIZE (go/biology_seqkit/seqkit-v2.8.2/golang-sys-v0.6.0_GH0.tar.gz) = 1434234
+TIMESTAMP = 1730758967
+SHA256 (go/biology_seqkit/seqkit-v2.9.0/v2.9.0.mod) = 13322621d4d5cc45785b0acb9170f81086c89d6b2a0ca98c926bc48e5d54f85f
+SIZE (go/biology_seqkit/seqkit-v2.9.0/v2.9.0.mod) = 2514
+SHA256 (go/biology_seqkit/seqkit-v2.9.0/v2.9.0.zip) = b0d867a592429d63766f576f8f7a520f9114e157d4c6f07c722a86e24ec8204c
+SIZE (go/biology_seqkit/seqkit-v2.9.0/v2.9.0.zip) = 34092924
+SHA256 (go/biology_seqkit/seqkit-v2.9.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
+SIZE (go/biology_seqkit/seqkit-v2.9.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234