diff --git a/biology/py-pysam/Makefile b/biology/py-pysam/Makefile
index d35d723e2075..b44f4e73a7f2 100644
--- a/biology/py-pysam/Makefile
+++ b/biology/py-pysam/Makefile
@@ -1,27 +1,27 @@
 PORTNAME=	pysam
-DISTVERSION=	0.21.0
+DISTVERSION=	0.22.0
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python module for reading, manipulating and writing genomic data sets
 WWW=		https://github.com/pysam-developers/pysam
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 LIB_DEPENDS=	libhts.so:biology/htslib
 
 USES=		gmake python:3.8-3.9
 USE_PYTHON=	distutils autoplist cython cython_run
 
 BINARY_ALIAS=	make=${GMAKE}
 MAKE_ENV=	HTSLIB_MODE=external \
 		HTSLIB_LIBRARY_DIR=${LOCALBASE}/lib \
 		HTSLIB_INCLUDE_DIR=${LOCALBASE}/include
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pysam/lib*${PYTHON_EXT_SUFFIX}.so
 
 .include <bsd.port.mk>
diff --git a/biology/py-pysam/distinfo b/biology/py-pysam/distinfo
index 1c9903177cca..24a863205256 100644
--- a/biology/py-pysam/distinfo
+++ b/biology/py-pysam/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1686950070
-SHA256 (pysam-0.21.0.tar.gz) = 5c9645ddd87668e36ff0a1966391e26f9c403bf85b1bc06c53fe2fcd592da2ce
-SIZE (pysam-0.21.0.tar.gz) = 4052537
+TIMESTAMP = 1696579895
+SHA256 (pysam-0.22.0.tar.gz) = ab7a46973cf0ab8c6ac327f4c3fb67698d7ccbeef8631a716898c6ba01ef3e45
+SIZE (pysam-0.22.0.tar.gz) = 4631254