diff --git a/biology/minimap2/Makefile b/biology/minimap2/Makefile
index 2ab83948882d..d403413cc324 100644
--- a/biology/minimap2/Makefile
+++ b/biology/minimap2/Makefile
@@ -1,26 +1,25 @@
 PORTNAME=		minimap2
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.25
-PORTREVISION=		1
+DISTVERSION=		2.27
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Pairwise aligner for genomic and spliced nucleotide sequences
 WWW=		https://github.com/lh3/minimap2
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 BUILD_DEPENDS=	simde>0:devel/simde
 
 USES=		gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	lh3
 
 ALL_TARGET=	extra
 INSTALL_TARGET=	install-strip
 
 MAKEFILE=	Makefile.simde
 
 .include <bsd.port.mk>
diff --git a/biology/minimap2/distinfo b/biology/minimap2/distinfo
index 7c79ce9501e2..5a1bf73dfa7d 100644
--- a/biology/minimap2/distinfo
+++ b/biology/minimap2/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1682729894
-SHA256 (lh3-minimap2-v2.25_GH0.tar.gz) = 9742ff0be01e51ea7d65f70c01d1344eee6f0d7b135359e0c00aec30fb74ac38
-SIZE (lh3-minimap2-v2.25_GH0.tar.gz) = 257574
+TIMESTAMP = 1710332275
+SHA256 (lh3-minimap2-v2.27_GH0.tar.gz) = ca9ceb07e3b388858ebc2d7d91a6c74e996659402d16aa759ecedd63588b1ef7
+SIZE (lh3-minimap2-v2.27_GH0.tar.gz) = 259736
diff --git a/biology/minimap2/files/patch-Makefile.simde b/biology/minimap2/files/patch-Makefile.simde
index faef217fbdab..5d5e0ccf482c 100644
--- a/biology/minimap2/files/patch-Makefile.simde
+++ b/biology/minimap2/files/patch-Makefile.simde
@@ -1,66 +1,66 @@
---- Makefile.simde.orig	2023-04-25 16:44:52 UTC
+--- Makefile.simde.orig	2021-11-18 22:11:48 UTC
 +++ Makefile.simde
 @@ -1,13 +1,26 @@
 -CFLAGS=		-g -Wall -O2 -Wc++-compat #-Wextra
 -CPPFLAGS=	-DHAVE_KALLOC -DUSE_SIMDE -DSIMDE_ENABLE_NATIVE_ALIASES
 -INCLUDES=	-Ilib/simde
 -OBJS=		kthread.o kalloc.o misc.o bseq.o sketch.o sdust.o options.o index.o lchain.o align.o hit.o map.o format.o pe.o seed.o esterr.o splitidx.o \
 -			ksw2_extz2_simde.o ksw2_extd2_simde.o ksw2_exts2_simde.o ksw2_ll_simde.o
 +
 +# Makefile patches rejected by upstream
 +
 +CFLAGS?=	-g -Wall -O2
 +CFLAGS+=	-Wc++-compat -DUSE_SIMDE -DSIMDE_ENABLE_NATIVE_ALIASES -D__SSE2__
 +CPPFLAGS?=	-DHAVE_KALLOC
 +OBJS=		kthread.o kalloc.o misc.o bseq.o sketch.o sdust.o options.o \
 +		index.o lchain.o align.o hit.o map.o format.o pe.o seed.o \
 +		esterr.o splitidx.o ksw2_extz2_simde.o ksw2_extd2_simde.o \
 +		ksw2_exts2_simde.o ksw2_ll_simde.o
  PROG=		minimap2
  PROG_EXTRA=	sdust minimap2-lite
  LIBS=		-lm -lz -lpthread
  
 +PREFIX?=	/usr/local
-+MAN1DIR?=	${PREFIX}/share/man/man1
++MAN1DIR?=	${PREFIX}/man/man1
 +DATADIR?=	${PREFIX}/share/minimap2
  
 +MKDIR?=		mkdir
 +CP?=		cp	
 +INSTALL?=	install
 +STRIP?=		strip
 +
  ifneq ($(arm_neon),) # if arm_neon is defined
  ifeq ($(aarch64),)   #if aarch64 is not defined
  	CFLAGS+=-D_FILE_OFFSET_BITS=64 -mfpu=neon -fsigned-char
-@@ -26,7 +39,7 @@ endif
+@@ -26,7 +39,7 @@ ifneq ($(tsan),)
  	LIBS+=-fsanitize=thread
  endif
  
 -.PHONY:all extra clean depend
 +.PHONY:all extra install install-strip clean depend
  .SUFFIXES:.c .o
  
  .c.o:
 @@ -61,6 +74,22 @@ ksw2_exts2_simde.o:ksw2_exts2_sse.c ksw2.h kalloc.h
  		$(CC) -c $(CFLAGS) -msse4.1 $(CPPFLAGS) $(INCLUDES) $< -o $@
  
  # other non-file targets
 +
 +
 +install: all
 +	${MKDIR} -p ${DESTDIR}${PREFIX}/bin
 +	${MKDIR} -p ${DESTDIR}${MAN1DIR}
 +	${MKDIR} -p ${DESTDIR}${DATADIR}
 +	${INSTALL} -c minimap2 ${DESTDIR}${PREFIX}/bin
 +	${INSTALL} -c minimap2-lite ${DESTDIR}${PREFIX}/bin
 +	${INSTALL} -c sdust ${DESTDIR}${PREFIX}/bin
 +	${INSTALL} -c minimap2.1 ${DESTDIR}${MAN1DIR}
 +	${CP} -R test ${DESTDIR}${DATADIR}
 +
 +install-strip: install
 +	${STRIP} ${DESTDIR}${PREFIX}/bin/minimap2
 +	${STRIP} ${DESTDIR}${PREFIX}/bin/minimap2-lite
 +	${STRIP} ${DESTDIR}${PREFIX}/bin/sdust
  
  clean:
  		rm -fr gmon.out *.o a.out $(PROG) $(PROG_EXTRA) *~ *.a *.dSYM build dist mappy*.so mappy.c python/mappy.c mappy.egg*
diff --git a/biology/minimap2/pkg-plist b/biology/minimap2/pkg-plist
index 742d1eb1b3f7..1d11d4c99c2f 100644
--- a/biology/minimap2/pkg-plist
+++ b/biology/minimap2/pkg-plist
@@ -1,10 +1,10 @@
 bin/minimap2
 bin/minimap2-lite
 bin/sdust
-share/man/man1/minimap2.1.gz
+man/man1/minimap2.1.gz
 %%DATADIR%%/test/MT-human.fa
 %%DATADIR%%/test/MT-orang.fa
 %%DATADIR%%/test/q-inv.fa
 %%DATADIR%%/test/q2.fa
 %%DATADIR%%/test/t-inv.fa
 %%DATADIR%%/test/t2.fa