diff --git a/biology/py-python-libsbml/Makefile b/biology/py-python-libsbml/Makefile
index cfb57bd01762..286dd1614dd6 100644
--- a/biology/py-python-libsbml/Makefile
+++ b/biology/py-python-libsbml/Makefile
@@ -1,26 +1,34 @@
 PORTNAME=	python-libsbml
-DISTVERSION=	5.20.4
-PORTREVISION=	1
+DISTVERSION=	5.21.1
 CATEGORIES=	biology python
 #MASTER_SITES=	PYPI # no tarballs on PYPI
 MASTER_SITES=	https://github.com/sbmlteam/python-libsbml/releases/download/v${DISTVERSION}/
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	LibSBML Python API
 WWW=		https://sbml.org/Main_Page
 
 LICENSE=	LGPL21
 
 BUILD_DEPENDS=	cmake:devel/cmake-core \
 		swig:devel/swig
 
 USES=		python
-USE_PYTHON=	autoplist distutils # autoplist produces incomplete plist, missing files are in pkg-plist. Reported to the upstream author fbergman@caltech.edu on 2021-08-17
+USE_PYTHON=	autoplist distutils pytest # autoplist produces incomplete plist, missing files are in pkg-plist. Reported to the upstream author fbergman@caltech.edu on 2021-08-17
+
+WRKSRC=		${WRKDIR}/python_libsbml-${DISTVERSION}
+
+TEST_WRKSRC=	${WRKSRC}/libsbml_source/src/bindings/python
 
 MAKE_ENV=	CMAKE_BUILD_PARALLEL_LEVEL=${MAKE_JOBS_NUMBER}
 
+do-test:
+	cd ${TEST_WRKSRC} && ${PYTHON_CMD} test.py -p ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}
+
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/libsbml/_libsbml*.so
 
+# tests as of 5.21.1: Ran 1819 tests in 0.078s
+
 .include <bsd.port.mk>
diff --git a/biology/py-python-libsbml/distinfo b/biology/py-python-libsbml/distinfo
index 26af45c03fd7..978825b1a053 100644
--- a/biology/py-python-libsbml/distinfo
+++ b/biology/py-python-libsbml/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1731400774
-SHA256 (python-libsbml-5.20.4.tar.gz) = c9d57cb81661eec7d9ec3a0efa463999d6cd389418dfdbeea764664f3a44617a
-SIZE (python-libsbml-5.20.4.tar.gz) = 48841959
+TIMESTAMP = 1777547689
+SHA256 (python-libsbml-5.21.1.tar.gz) = 378077e37a1b5cd32d8c33bf845b23c22b7a6915d217d7ab811e71e022f982ea
+SIZE (python-libsbml-5.21.1.tar.gz) = 41854207