diff --git a/biology/py-macs3/Makefile b/biology/py-macs3/Makefile
index 6ad3d9000471..b51819da5c62 100644
--- a/biology/py-macs3/Makefile
+++ b/biology/py-macs3/Makefile
@@ -1,49 +1,52 @@
 PORTNAME=		MACS3
 DISTVERSIONPREFIX=	v
 DISTVERSION=		3.0.1
 CATEGORIES=		biology python
 PKGNAMEPREFIX=		${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Peak caller aimed at transcription factor binding sites
 WWW=		https://github.com/taoliu/MACS
 
 LICENSE=	BSD3CLAUSE
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
+NOT_FOR_ARCHS=	i386
+NOT_FOR_ARCHS_REASON_i386=Requires _Float16
+
 BUILD_DEPENDS=	${PYTHON_PKGNAMEPREFIX}wheel>0:devel/py-wheel@${PY_FLAVOR} \
 		${PYNUMPY} \
 		simde>0:devel/simde \
 		${PYTHON_PKGNAMEPREFIX}cykhash>0:devel/py-cykhash@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}hmmlearn>0:math/py-hmmlearn@${PY_FLAVOR}
 RUN_DEPENDS=	${PYNUMPY} \
 		${PYTHON_PKGNAMEPREFIX}cykhash>0:devel/py-cykhash@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}hmmlearn>0:math/py-hmmlearn@${PY_FLAVOR}
 TEST_DEPENDS=	${PYTHON_PKGNAMEPREFIX}pytest>0:devel/py-pytest@${PY_FLAVOR} \
 		bash:shells/bash
 
 USES=		localbase perl5 python shebangfix
 USE_GITHUB=	yes
 USE_PERL5=	test
 USE_PYTHON=	autoplist concurrent cython pep517
 
 GH_ACCOUNT=	macs3-project
 GH_PROJECT=	MACS
 SHEBANG_FILES=	test/cmdlinetest* test/prockreport test/*.py test/pyprofile*
 
 .include <bsd.port.options.mk>
 
 .if ${OPSYS} == FreeBSD && ${OSVERSION} < 1302509
 IGNORE=		does not run properly on FreeBSD 13.2 and earlier due to use of dynamically loaded libc by py-threadpoolctl.  Please upgrade to a newer FreeBSD or use py-macs2 instead.  See PR 276231
 .endif
 
 post-stage:
 	${STRIP_CMD} ${STAGEDIR}${PYTHON_SITELIBDIR}/MACS3/*/*.so
 
 # FIXME: Some tests fail due to a py-threadpoolctl issue
 # https://github.com/joblib/threadpoolctl/pull/148
 do-test: deinstall reinstall
 	@${RM} -rf ${WRKSRC}/temp
 	@cd ${WRKSRC}/test && ./cmdlinetest tag
 
 .include <bsd.port.mk>
diff --git a/biology/py-macs3/distinfo b/biology/py-macs3/distinfo
index b06631987784..b742493c53a1 100644
--- a/biology/py-macs3/distinfo
+++ b/biology/py-macs3/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1708785426
+TIMESTAMP = 1713397031
 SHA256 (macs3-project-MACS-v3.0.1_GH0.tar.gz) = 8a2f5b2dccfc255ea2fd5e54e0d6aa9c12f5ce83762b17b779ce2546ade860ce
 SIZE (macs3-project-MACS-v3.0.1_GH0.tar.gz) = 197400439