diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index 4551cddb0836..62698efd5be6 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,19 +1,19 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	0.16.0
+DISTVERSION=	0.16.1
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 
 GO_MODULE=	github.com/shenwei356/seqkit
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index b24424ffad25..3f7438fc993f 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,5 +1,5 @@
-TIMESTAMP = 1618605249
-SHA256 (go/biology_seqkit/seqkit-v0.16.0/v0.16.0.mod) = 2b29e40d7d4b891b1ece058363b9b4f8b3ba6385aa2855fb8d5f3df25bc83057
-SIZE (go/biology_seqkit/seqkit-v0.16.0/v0.16.0.mod) = 1211
-SHA256 (go/biology_seqkit/seqkit-v0.16.0/v0.16.0.zip) = 91fe05c0ead7b5dfafea7a0b3793583a3190befdcf3165735bf152182d0b0b11
-SIZE (go/biology_seqkit/seqkit-v0.16.0/v0.16.0.zip) = 29977829
+TIMESTAMP = 1621489008
+SHA256 (go/biology_seqkit/seqkit-v0.16.1/v0.16.1.mod) = 2b29e40d7d4b891b1ece058363b9b4f8b3ba6385aa2855fb8d5f3df25bc83057
+SIZE (go/biology_seqkit/seqkit-v0.16.1/v0.16.1.mod) = 1211
+SHA256 (go/biology_seqkit/seqkit-v0.16.1/v0.16.1.zip) = 1127a5b026fbe962b6c91647a702dfa653e76005fa71c6f15d46902a137066da
+SIZE (go/biology_seqkit/seqkit-v0.16.1/v0.16.1.zip) = 29978026