diff --git a/biology/mashmap/Makefile b/biology/mashmap/Makefile
index b1be9f3f6461..510ea1f1c2ea 100644
--- a/biology/mashmap/Makefile
+++ b/biology/mashmap/Makefile
@@ -1,31 +1,31 @@
 PORTNAME=	mashmap
 DISTVERSIONPREFIX=	v
-DISTVERSION=	3.0.6
-PORTREVISION=	1
+DISTVERSION=	3.1.0
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Fast approximate aligner for long DNA sequences
 WWW=		https://github.com/marbl/MashMap
 
 LICENSE=	PD
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 BROKEN_i386=	compilation fails: fatal error: unknown type name '__uint128_t'
 
-LIB_DEPENDS=	libgsl.so:math/gsl
+LIB_DEPENDS=	libgsl.so:math/gsl \
+		libhts.so:biology/htslib
 
 USES=		cmake compiler:c++17-lang localbase:ldflags
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	marbl
 GH_PROJECT=	MashMap
 
 OPTIONS_DEFINE=		NATIVE
 
 NATIVE_CMAKE_BOOL=	OPTIMIZE_FOR_NATIVE
 
 PLIST_FILES=	bin/mashmap \
 		bin/mashmap-align
 
 .include <bsd.port.mk>
diff --git a/biology/mashmap/distinfo b/biology/mashmap/distinfo
index 7cbf1e62148e..363695274044 100644
--- a/biology/mashmap/distinfo
+++ b/biology/mashmap/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1689039441
-SHA256 (marbl-MashMap-v3.0.6_GH0.tar.gz) = 53d1b4efad6650d8efbc28325637d1bdacd108eaad70fcc28e927f40ac5c2112
-SIZE (marbl-MashMap-v3.0.6_GH0.tar.gz) = 234093
+TIMESTAMP = 1692677659
+SHA256 (marbl-MashMap-v3.1.0_GH0.tar.gz) = ad1e41691faec3784aaaebee99db9df79dea9db82de7a2140e86d50bfec965f4
+SIZE (marbl-MashMap-v3.1.0_GH0.tar.gz) = 25919246