diff --git a/biology/htslib/Makefile b/biology/htslib/Makefile
index 5b3c5908f428..aa4f02a37d15 100644
--- a/biology/htslib/Makefile
+++ b/biology/htslib/Makefile
@@ -1,42 +1,44 @@
 PORTNAME=	htslib
-DISTVERSION=	1.22
+DISTVERSION=	1.23.1
 CATEGORIES=	biology devel
 # Github generated distfiles are incomplete, so use custom tarball
 MASTER_SITES=	https://github.com/samtools/htslib/releases/download/${DISTVERSION}/
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	C library for high-throughput sequencing data formats
 WWW=		https://www.htslib.org/
 
 LICENSE=	BSD3CLAUSE MIT
 LICENSE_COMB=	multi
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 LIB_DEPENDS=	libdeflate.so:archivers/libdeflate
 TEST_DEPENDS=	bash:shells/bash
 
 USES=		autoreconf cpe gmake localbase:ldflags perl5 shebangfix tar:bzip2
 USE_PERL5=	test
 USE_LDCONFIG=	yes
 
 GNU_CONFIGURE=		yes
 GNU_CONFIGURE_MANPREFIX=${PREFIX}/share
 MAKE_ARGS_armv6=	HTS_HAVE_NEON=
 MAKE_ARGS_armv7=	HTS_HAVE_NEON=
 MAKE_ARGS=		${MAKE_ARGS_${ARCH}}
 PATHFIX_MAKEFILEIN=	Makefile
 SHEBANG_FILES=		test/*.pl
 
-OPTIONS_DEFINE=		CURL
-OPTIONS_DEFAULT=	CURL
+OPTIONS_DEFINE=		CURL PLUGINS
+OPTIONS_DEFAULT=	CURL PLUGINS
 
 CURL_DESC=		Use libcurl for network file access (HTTPS and S3 support).
 CURL_USES=		ssl
 CURL_LIB_DEPENDS=	libcurl.so:ftp/curl
 CURL_CONFIGURE_ENABLE=	libcurl
+PLUGIN_DESC=		Enable external plugins for exotic file access, etc.
+PLUGIN_CONFIGURE_ON=	plugins
 
 PLIST_SUB=	PORTVERSION="${PORTVERSION}"
 
 TEST_TARGET=	test
 
 .include <bsd.port.mk>
diff --git a/biology/htslib/distinfo b/biology/htslib/distinfo
index fbe2d5f9d13e..257fe618a524 100644
--- a/biology/htslib/distinfo
+++ b/biology/htslib/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1748951113
-SHA256 (htslib-1.22.tar.bz2) = 6250c1df297db477516e60ac8df45ed75a652d1f25b0f37f12f5b17269eafde9
-SIZE (htslib-1.22.tar.bz2) = 4830206
+TIMESTAMP = 1774783712
+SHA256 (htslib-1.23.1.tar.bz2) = f8a3f36effeec38f043c53ab1f2d9ed45064f14205c5ef8e3c815763b90803c4
+SIZE (htslib-1.23.1.tar.bz2) = 4987046