diff --git a/biology/locarna/Makefile b/biology/locarna/Makefile
index 7f77a818b5fe..58e638d180b3 100644
--- a/biology/locarna/Makefile
+++ b/biology/locarna/Makefile
@@ -1,39 +1,38 @@
 PORTNAME=	locarna
-DISTVERSION=	2.0.0
-PORTREVISION=	2
+DISTVERSION=	2.0.1
 CATEGORIES=	biology
 MASTER_SITES=	https://github.com/s-will/LocARNA/releases/download/v${DISTVERSION}/
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	LocARNA provides several tools for the structural analysis of RNA
 WWW=		https://github.com/s-will/LocARNA
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 BUILD_DEPENDS=	${LOCALBASE}/lib/libRNA.a:biology/viennarna
 LIB_DEPENDS=	libgmp.so:math/gmp \
 		libgsl.so:math/gsl \
 		libmpfr.so:math/mpfr
 TEST_DEPENDS=	bash:shells/bash \
 		catch>0:devel/catch
 
 USES=		autoreconf gmake libtool perl5 pkgconfig shebangfix
 USE_LDCONFIG=	yes
 USE_CXXSTD=	c++14 # workaround for https://github.com/s-will/LocARNA/issues/82 (LocARNA uses the removed STL method)
 
 CXXFLAGS+=	-I${LOCALBASE}/include/catch2 # use external catch
 
 SHEBANG_FILES=	src/Utils/locarna_mcc \
 		src/Tests/test_programs
 
 GNU_CONFIGURE=	yes
 GNU_CONFIGURE_MANPREFIX=	${PREFIX}/share
 
 INSTALL_TARGET=	install-strip
 TEST_TARGET=	check # one test fails, see https://github.com/s-will/LocARNA/issues/81
 
 post-extract: # unbundle catch, see https://github.com/s-will/LocARNA/issues/80
 	@${RM} ${WRKSRC}/src/Tests/catch.hpp
 
 .include <bsd.port.mk>
diff --git a/biology/locarna/distinfo b/biology/locarna/distinfo
index 1b872dee9ea6..65f61fd43d1e 100644
--- a/biology/locarna/distinfo
+++ b/biology/locarna/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1689540878
-SHA256 (locarna-2.0.0.tar.gz) = 3be0d2cac7f7451f8b741983e22803b546de2074d837b425fb1b69ccc718ebbc
-SIZE (locarna-2.0.0.tar.gz) = 3049455
+TIMESTAMP = 1778048412
+SHA256 (locarna-2.0.1.tar.gz) = 1f50748d2f8f4bf5888394154d366e0eed8d4e20a3cd0da8b33e200aa1d9edf7
+SIZE (locarna-2.0.1.tar.gz) = 3135134