diff --git a/biology/py-py2bit/Makefile b/biology/py-py2bit/Makefile
index 0450a02e35c8..8d6ec9f6c2a0 100644
--- a/biology/py-py2bit/Makefile
+++ b/biology/py-py2bit/Makefile
@@ -1,18 +1,21 @@
 PORTNAME=	py2bit
-DISTVERSION=	0.3.0
-PORTREVISION=	1
+DISTVERSION=	1.0.1
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Python interface for 2bit packed nucleotide files
 WWW=		https://github.com/deeptools/py2bit
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 USES=		python
+# Can't switch to pep517 yet: Requires setuptools>=74.1
 USE_PYTHON=	autoplist distutils
 
+post-stage:
+	${STRIP_CMD} ${STAGEDIR}${PYTHON_SITELIBDIR}/py2bit.cpython-311.so
+
 .include <bsd.port.mk>
diff --git a/biology/py-py2bit/distinfo b/biology/py-py2bit/distinfo
index a797a50f82d2..5aa3c295ea8e 100644
--- a/biology/py-py2bit/distinfo
+++ b/biology/py-py2bit/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1617287956
-SHA256 (py2bit-0.3.0.tar.gz) = 450555c40cba66957ac8c9a4b6afb625fb34c4bb41638de78c87661ff8b682ef
-SIZE (py2bit-0.3.0.tar.gz) = 16010
+TIMESTAMP = 1777141077
+SHA256 (py2bit-1.0.1.tar.gz) = 4972f85eb3844cdfba43eb54ab3c8349a0536e03dfd7db07ca8d3447285ad20c
+SIZE (py2bit-1.0.1.tar.gz) = 18765