diff --git a/net/py-trio/Makefile b/net/py-trio/Makefile
index 9254ae3efd0d..15718fd14ef5 100644
--- a/net/py-trio/Makefile
+++ b/net/py-trio/Makefile
@@ -1,44 +1,46 @@
 PORTNAME=	trio
-DISTVERSION=	0.32.0
+DISTVERSION=	0.33.0
 CATEGORIES=	net python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	ehaupt@FreeBSD.org
 COMMENT=	Library for async concurrency and I/O
 WWW=		https://pypi.org/project/trio/ \
 		https://github.com/python-trio/trio
 
 LICENSE=	APACHE20 MIT
 LICENSE_COMB=	dual
 LICENSE_FILE_APACHE20=	${WRKSRC}/LICENSE.APACHE2
 LICENSE_FILE_MIT=	${WRKSRC}/LICENSE.MIT
 
 BUILD_DEPENDS=	${PY_SETUPTOOLS} \
 		${PYTHON_PKGNAMEPREFIX}wheel>=0:devel/py-wheel@${PY_FLAVOR}
 RUN_DEPENDS=	${PY_EXCEPTIONGROUP} \
 		${PYTHON_PKGNAMEPREFIX}async_generator>0:devel/py-async_generator@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}attrs>=19.2.0:devel/py-attrs@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}cffi>=1.14:devel/py-cffi@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}idna>0:dns/py-idna@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}outcome>0:devel/py-outcome@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}sniffio>0:devel/py-sniffio@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}sortedcontainers>0:devel/py-sortedcontainers@${PY_FLAVOR}
 TEST_DEPENDS=	${PYTHON_PKGNAMEPREFIX}astor>=0:devel/py-astor@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}black>=0:devel/py-black@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}jedi>=0.18.0:devel/py-jedi@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}pyopenssl>=0:security/py-pyopenssl@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}pytest>=0:devel/py-pytest@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}ruff>=0:devel/py-ruff@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}trustme>=0:security/py-trustme@${PY_FLAVOR} \
 		pylint${PYTHON_PKGNAMESUFFIX}>=0:devel/pylint@${PY_FLAVOR}
 
 USES=		python
 USE_PYTHON=	autoplist pep517
 
+TEST_ENV=	PYTEST_DISABLE_PLUGIN_AUTOLOAD=1 \
+		PYTHONPATH=${WRKSRC}/src
 NO_ARCH=	yes
 
 do-test:
 	@cd ${TEST_WRKSRC} && ${SETENV} ${TEST_ENV} ${PYTHON_CMD} -m pytest -v -rs
 
 .include <bsd.port.mk>
diff --git a/net/py-trio/distinfo b/net/py-trio/distinfo
index ef579d85de7d..fb36dd35a456 100644
--- a/net/py-trio/distinfo
+++ b/net/py-trio/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1763108231
-SHA256 (trio-0.32.0.tar.gz) = 150f29ec923bcd51231e1d4c71c7006e65247d68759dd1c19af4ea815a25806b
-SIZE (trio-0.32.0.tar.gz) = 605323
+TIMESTAMP = 1771233379
+SHA256 (trio-0.33.0.tar.gz) = a29b92b73f09d4b48ed249acd91073281a7f1063f09caba5dc70465b5c7aa970
+SIZE (trio-0.33.0.tar.gz) = 605109