diff --git a/biology/py-pysam/Makefile b/biology/py-pysam/Makefile
index d93aa642d48d..2b86675e98b7 100644
--- a/biology/py-pysam/Makefile
+++ b/biology/py-pysam/Makefile
@@ -1,16 +1,16 @@
 PORTNAME=	pysam
-DISTVERSION=	0.16.0.1
+DISTVERSION=	0.18.0
 CATEGORIES=	biology python
 MASTER_SITES=	CHEESESHOP
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python module for reading, manipulating and writing genomic data sets
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 USES=		gmake python:3.6-3.9
 USE_PYTHON=	autoplist distutils cython cython_run
 
 .include <bsd.port.mk>
diff --git a/biology/py-pysam/distinfo b/biology/py-pysam/distinfo
index 102e94b395cc..b85394097108 100644
--- a/biology/py-pysam/distinfo
+++ b/biology/py-pysam/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1605468111
-SHA256 (pysam-0.16.0.1.tar.gz) = d428a9768691d5ea3c28cc52a949c920ae691aa4c110a8b7328dc4d165ef1ad6
-SIZE (pysam-0.16.0.1.tar.gz) = 3552537
+TIMESTAMP = 1639969328
+SHA256 (pysam-0.18.0.tar.gz) = 1d6d49a0b3c626fae410a93d4c80583a8b5ddaacc9b46a080b250dbcebd30a59
+SIZE (pysam-0.18.0.tar.gz) = 3780010