diff --git a/biology/subread/Makefile b/biology/subread/Makefile
index e94ede742f9a..a1d05133fe3d 100644
--- a/biology/subread/Makefile
+++ b/biology/subread/Makefile
@@ -1,25 +1,25 @@
 PORTNAME=		subread
-DISTVERSION=		2.0.8
+DISTVERSION=		2.1.0
 DISTVERSIONSUFFIX=	-source
 CATEGORIES=		biology
 MASTER_SITES=		SF/${PORTNAME}/${PORTNAME}-${PORTVERSION}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	High-performance read alignment, quantification and mutation discovery
 WWW=		https://subread.sourceforge.net/
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKDIR}/${DISTNAME}/LICENSE
 
 LIB_DEPENDS=	libsysinfo.so:devel/libsysinfo
 
 USES=		gmake localbase:ldflags
 
 WRKSRC_SUBDIR=	src
 
 MAKEFILE=	${FILESDIR}/Makefile.pkg
 INSTALL_TARGET=	install-strip
 
 CFLAGS+=	-fcommon
 
 .include <bsd.port.mk>
diff --git a/biology/subread/distinfo b/biology/subread/distinfo
index 7e6e9e5f7d23..5463062ccfa5 100644
--- a/biology/subread/distinfo
+++ b/biology/subread/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1733935409
-SHA256 (subread-2.0.8-source.tar.gz) = cb5dadf71c850f128a8347223fe0ce19e1c17e3959e9f3eba90e8090671f8570
-SIZE (subread-2.0.8-source.tar.gz) = 26074498
+TIMESTAMP = 1743112595
+SHA256 (subread-2.1.0-source.tar.gz) = fdf8a84cf145edab4984070e71595612c97ad3741afdb8d25f93dae7c8703433
+SIZE (subread-2.1.0-source.tar.gz) = 26090041