diff --git a/biology/py-cutadapt/Makefile b/biology/py-cutadapt/Makefile
index 8c86bee19024..3c2ac0802f5a 100644
--- a/biology/py-cutadapt/Makefile
+++ b/biology/py-cutadapt/Makefile
@@ -1,30 +1,30 @@
 PORTNAME=	cutadapt
-DISTVERSION=	4.9
+DISTVERSION=	5.0
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jadawin@FreeBSD.org
 COMMENT=	Trim adapters from high-throughput sequencing reads
 WWW=		https://github.com/marcelm/cutadapt/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 BUILD_DEPENDS=	${PYTHON_PKGNAMEPREFIX}setuptools-scm>0:devel/py-setuptools-scm@${PY_FLAVOR}
 RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}xopen>=1.6.0:archivers/py-xopen@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}dnaio>=1.2.0:biology/py-dnaio@${PY_FLAVOR}
 TEST_DEPENDS=	${PYTHON_PKGNAMEPREFIX}pytest>0:devel/py-pytest@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}pytest-timeout>0:devel/py-pytest-timeout@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}pytest-mock>0:devel/py-pytest-mock@${PY_FLAVOR}
 
 USES=		python
 USE_PYTHON=	autoplist concurrent cython distutils
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHON_SITELIBDIR}/cutadapt/*.so
 
 do-test:
 	@cd ${WRKSRC} && ${PYTHON_CMD} -m pytest
 
 .include <bsd.port.mk>
diff --git a/biology/py-cutadapt/distinfo b/biology/py-cutadapt/distinfo
index 8e356eb3ce14..1613aa2d6c1a 100644
--- a/biology/py-cutadapt/distinfo
+++ b/biology/py-cutadapt/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1718872674
-SHA256 (cutadapt-4.9.tar.gz) = da3b45775b07334d2e2580a7b154d19ea7e872f0da813bb1ac2a4da712bfc223
-SIZE (cutadapt-4.9.tar.gz) = 248837
+TIMESTAMP = 1736523245
+SHA256 (cutadapt-5.0.tar.gz) = cd66872b6635c068cf223f72fb0cc93b2454088a3e807a550bb36f717d25ae29
+SIZE (cutadapt-5.0.tar.gz) = 251297