diff --git a/biology/muscle/Makefile b/biology/muscle/Makefile
index f243953b2e0e..a88c642204a1 100644
--- a/biology/muscle/Makefile
+++ b/biology/muscle/Makefile
@@ -1,29 +1,25 @@
 PORTNAME=	muscle
 PORTVERSION=	5.1
 DISTVERSIONPREFIX=	v
+PORTREVISION=	1
 CATEGORIES=	biology
 
 MAINTAINER=	mzaki@e-mail.ne.jp
 COMMENT=	MUltiple Sequence Comparison by Log-Expectation
 WWW=		https://www.drive5.com/muscle/
 
 LICENSE=	GPLv3
 
-BUILD_DEPENDS=	bash:shells/bash
-
 USES=		gmake
-USE_GCC=	yes
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	rcedgar
 ALL_TARGET=	${OPSYS}/${PORTNAME}
-BINARY_ALIAS=	g++=g++14
 WRKSRC_SUBDIR=	src
 
 PLIST_FILES=	bin/muscle
 
 do-install:
-	cd ${WRKSRC} && \
-	${INSTALL} ${OPSYS}/muscle ${STAGEDIR}${PREFIX}/bin/
+	${INSTALL_PROGRAM} ${WRKSRC}/${OPSYS}/muscle ${STAGEDIR}${PREFIX}/bin/
 
 .include <bsd.port.mk>
diff --git a/biology/muscle/files/patch-src__Makefile b/biology/muscle/files/patch-src__Makefile
new file mode 100644
index 000000000000..3fbc3ebfed80
--- /dev/null
+++ b/biology/muscle/files/patch-src__Makefile
@@ -0,0 +1,20 @@
+--- Makefile.orig	2022-01-13 23:29:59.000000000 +0000
++++ Makefile	2026-07-21 12:39:09.844672000 +0000
+@@ -19,7 +19,7 @@
+ 
+ CPPFLAGS := $(CPPFLAGS) -DNDEBUG -pthread
+ 
+-CXX := g++
++CXX ?= g++
+ ifeq ($(OS),Darwin)
+ 	CXX := g++-11
+ endif
+@@ -44,7 +44,7 @@
+ 	strip $(OS)/muscle
+ 
+ gitver.txt : $(SRCS)
+-	bash ./gitver.bash
++	sh ./gitver.bash
+ 
+ $(OS)/ :
+ 	mkdir -p $(OS)/