diff --git a/biology/diamond/Makefile b/biology/diamond/Makefile
index c63fcb6e504d..62604d6f0e97 100644
--- a/biology/diamond/Makefile
+++ b/biology/diamond/Makefile
@@ -1,24 +1,24 @@
 PORTNAME=	diamond
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.2.4
+DISTVERSION=	2.2.5
 CATEGORIES=	biology
 
 MAINTAINER=	jrm@FreeBSD.org
 COMMENT=	BLAST-compatible local sequence aligner
 WWW=		http://ab.inf.uni-tuebingen.de/software/diamond/ \
 		https://github.com/bbuchfink/diamond
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 ONLY_FOR_ARCHS=	aarch64 amd64 powerpc64 powerpc64le
 
 USES=		cmake localbase sqlite
 USE_GITHUB=	yes
 GH_ACCOUNT=	bbuchfink
 
 TEST_TARGET=	test
 
 PLIST_FILES=	bin/diamond
 
 .include <bsd.port.mk>
diff --git a/biology/diamond/distinfo b/biology/diamond/distinfo
index 823bda4b4b8c..f80092a45ef9 100644
--- a/biology/diamond/distinfo
+++ b/biology/diamond/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1785212282
-SHA256 (bbuchfink-diamond-v2.2.4_GH0.tar.gz) = 11c593677b67ef541095122c243eed384bb9933da12fffc676ad7b35a3e4dc44
-SIZE (bbuchfink-diamond-v2.2.4_GH0.tar.gz) = 15147435
+TIMESTAMP = 1785776010
+SHA256 (bbuchfink-diamond-v2.2.5_GH0.tar.gz) = ca58297af1c60e02a36363f11d161a27f5bd4fed8b172cc56fab65805f37056a
+SIZE (bbuchfink-diamond-v2.2.5_GH0.tar.gz) = 15169124
diff --git a/biology/diamond/files/patch-src_basic_hssp.cpp b/biology/diamond/files/patch-src_basic_hssp.cpp
new file mode 100644
index 000000000000..ed351593e4a6
--- /dev/null
+++ b/biology/diamond/files/patch-src_basic_hssp.cpp
@@ -0,0 +1,10 @@
+--- src/basic/hssp.cpp.orig	2026-08-04 03:42:17 UTC
++++ src/basic/hssp.cpp
+@@ -356,6 +356,7 @@ Hsp::Hsp(const ApproxHsp& h, Loc qlen, Loc tlen) :
+ 
+ Hsp::Hsp(const ApproxHsp& h, Loc qlen, Loc tlen) :
+ 	backtraced(true),
++	seed_only(false),
+ 	score(h.score),
+ 	frame(0),
+ 	length(h.query_range.length()),
diff --git a/biology/diamond/files/patch-src_util_io_file.cpp b/biology/diamond/files/patch-src_util_io_file.cpp
deleted file mode 100644
index 110aff8b7e35..000000000000
--- a/biology/diamond/files/patch-src_util_io_file.cpp
+++ /dev/null
@@ -1,11 +0,0 @@
---- src/util/io/file.cpp.orig	2026-07-13 11:36:09 UTC
-+++ src/util/io/file.cpp
-@@ -21,6 +21,8 @@ along with this program.  If not, see <http://www.gnu.
- #ifdef _WIN32
- #include <io.h>
- #include <fcntl.h>
-+#else
-+#include <unistd.h> // unlink
- #endif
- #include <string.h> // strerror
- #include "file.h"