diff --git a/biology/py-pyrodigal/Makefile b/biology/py-pyrodigal/Makefile
index 2beef1a2708c..f42efb8ae016 100644
--- a/biology/py-pyrodigal/Makefile
+++ b/biology/py-pyrodigal/Makefile
@@ -1,28 +1,28 @@
 PORTNAME=	pyrodigal
-DISTVERSION=	2.0.3
+DISTVERSION=	2.1.0
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python binding for Prodigal, an ORF finder for genomes and metagenomes
 WWW=		https://github.com/althonos/pyrodigal
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 USES=		python:3.7+
 USE_PYTHON=	distutils cython concurrent autoplist pytest
 
 TEST_ENV=	${MAKE_ENV} PYTHONPATH=${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}
 
 post-build:
 	@cd ${BUILD_WRKSRC} && ${SETENV} ${MAKE_ENV} ${PYTHON_CMD} setup.py build_ext --inplace
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pyrodigal/_pyrodigal${PYTHON_EXT_SUFFIX}.so
 
 do-test:
 	@cd ${TEST_WRKSRC} && ${SETENV} ${TEST_ENV} ${PYTHON_CMD} -m unittest discover -vv
 
 .include <bsd.port.mk>
diff --git a/biology/py-pyrodigal/distinfo b/biology/py-pyrodigal/distinfo
index a693f5c71fb0..6af6b60b4096 100644
--- a/biology/py-pyrodigal/distinfo
+++ b/biology/py-pyrodigal/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1671688245
-SHA256 (pyrodigal-2.0.3.tar.gz) = 7d101495115683523271cd5541d1a1f357eb699d7f382fb5dc1850a91a9220d1
-SIZE (pyrodigal-2.0.3.tar.gz) = 3076043
+TIMESTAMP = 1679243745
+SHA256 (pyrodigal-2.1.0.tar.gz) = a4c07baa2ceebb052d8b65749c30b41fec998bb7f3600da1979fb0b5f328a34e
+SIZE (pyrodigal-2.1.0.tar.gz) = 2617257