diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index b1cce8594757..4c4ee6869fe2 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,31 +1,30 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.5.0
-PORTREVISION=	1
+DISTVERSION=	2.5.1
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 WWW=		https://bioinf.shenwei.me/seqkit/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 USES=		go:modules
 USE_GITHUB=	nodefault
 GH_TUPLE=	golang:sys:v0.6.0:sys
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 pre-patch:
 	${RM} -r ${WRKSRC}/vendor/golang.org/x/sys
 	${LN} -s ${WRKDIR}/sys-* ${WRKSRC}/vendor/golang.org/x/sys
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index fe0714901e3b..7fac91c8f1e3 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,7 +1,7 @@
-TIMESTAMP = 1689568589
-SHA256 (go/biology_seqkit/seqkit-v2.5.0/v2.5.0.mod) = 9697f35b9c9941df33360b60f42ff021857a291e62f2e43b98749849027b63d2
-SIZE (go/biology_seqkit/seqkit-v2.5.0/v2.5.0.mod) = 2508
-SHA256 (go/biology_seqkit/seqkit-v2.5.0/v2.5.0.zip) = 382aa903fa80cffdba2aa7e2d2adb02718e2c4cdb0dee060e0695ba10471f212
-SIZE (go/biology_seqkit/seqkit-v2.5.0/v2.5.0.zip) = 33915135
-SHA256 (go/biology_seqkit/seqkit-v2.5.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
-SIZE (go/biology_seqkit/seqkit-v2.5.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234
+TIMESTAMP = 1691553940
+SHA256 (go/biology_seqkit/seqkit-v2.5.1/v2.5.1.mod) = 9697f35b9c9941df33360b60f42ff021857a291e62f2e43b98749849027b63d2
+SIZE (go/biology_seqkit/seqkit-v2.5.1/v2.5.1.mod) = 2508
+SHA256 (go/biology_seqkit/seqkit-v2.5.1/v2.5.1.zip) = fd572ed7f71e204fb5691cb25119615e4149404ab64dc2a3449c1fe064bc6caf
+SIZE (go/biology_seqkit/seqkit-v2.5.1/v2.5.1.zip) = 33915222
+SHA256 (go/biology_seqkit/seqkit-v2.5.1/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
+SIZE (go/biology_seqkit/seqkit-v2.5.1/golang-sys-v0.6.0_GH0.tar.gz) = 1434234