diff --git a/biology/hisat2/Makefile b/biology/hisat2/Makefile
index da8449466d81..affd61bf35cf 100644
--- a/biology/hisat2/Makefile
+++ b/biology/hisat2/Makefile
@@ -1,30 +1,29 @@
 PORTNAME=		hisat2
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.2.1
-PORTREVISION=		2
+DISTVERSION=		2.2.2
 CATEGORIES=		biology perl5 python
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Alignment program for mapping next-generation sequencing reads
 WWW=		https://ccb.jhu.edu/software/hisat2/index.shtml
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64 powerpc64le riscv64
 ONLY_FOR_ARCHS_REASON=	64-bit code, some assembly language
 
 BUILD_DEPENDS=	simde>0:devel/simde
 
 USES=		gmake localbase perl5 python shebangfix
 USE_GITHUB=	yes
 
 SHEBANG_FILES=	hisat2 hisat2-build hisat2-inspect \
 		*.py */*.py */*/*.py */*/*/*.py \
 		scripts/*.sh evaluation/tests/HLA_novel/scripts/*.sh \
 		scripts/*.pl docs_jhu/*.pl
 GH_ACCOUNT=	DaehwanKimLab
 
 CFLAGS+=	-Wno-deprecated-declarations -Wno-char-subscripts -fsigned-char
 
 .include <bsd.port.mk>
diff --git a/biology/hisat2/distinfo b/biology/hisat2/distinfo
index 7cfdc88f2291..c9fa4c2d0d22 100644
--- a/biology/hisat2/distinfo
+++ b/biology/hisat2/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1637941870
-SHA256 (DaehwanKimLab-hisat2-v2.2.1_GH0.tar.gz) = f3f4f867d0a6b1f880d64efc19deaa5788c62050e0a4d614ce98b3492f702599
-SIZE (DaehwanKimLab-hisat2-v2.2.1_GH0.tar.gz) = 6761242
+TIMESTAMP = 1769696849
+SHA256 (DaehwanKimLab-hisat2-v2.2.2_GH0.tar.gz) = d3996d7bee30e38e51beb69c44b10461a4692e686487c465f9a20e3f54b6e815
+SIZE (DaehwanKimLab-hisat2-v2.2.2_GH0.tar.gz) = 6980416