diff --git a/biology/bio-mocha/Makefile b/biology/bio-mocha/Makefile
index 828b246380b0..c8c229801d1c 100644
--- a/biology/bio-mocha/Makefile
+++ b/biology/bio-mocha/Makefile
@@ -1,49 +1,48 @@
 PORTNAME=	bio-mocha
-DISTVERSION=	1.20
-PORTREVISION=	1
+DISTVERSION=	1.22
 CATEGORIES=	biology
 MASTER_SITES=	https://software.broadinstitute.org/software/mocha/
 # Get this right, or ports will download bcftools in it's place via tuple
-DISTFILES+=	${PORTNAME}_${DISTVERSION}-20240505.tar.gz
+DISTFILES+=	${PORTNAME}_${DISTVERSION}-20250819.tar.gz
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Bcftools plugin for mosaic chromosomal alteration analysis
 WWW=		https://software.broadinstitute.org/software/mocha/
 
 LICENSE=	MIT
 
-BUILD_DEPENDS=	bash:shells/bash htslib>=1.22:biology/htslib
+BUILD_DEPENDS=	bash:shells/bash htslib>=1.24:biology/htslib
 LIB_DEPENDS=	libhts.so:biology/htslib
 RUN_DEPENDS=	bcftools>=${PORTVERSION}:biology/bcftools
 
 USES=		autoreconf gmake localbase perl5 python:env shebangfix
 USE_GITHUB=	yes
 USE_PERL5=	test
 
 GH_ACCOUNT=	samtools
 GH_PROJECT=	bcftools
-GH_TAGNAME=	1.22
+GH_TAGNAME=	1.24
 GNU_CONFIGURE=	yes
 SHEBANG_FILES=	misc/* test/test.pl
 
 post-extract:
 	@${MV} ${WRKDIR}/*.c ${WRKDIR}/*.h ${WRKSRC}/plugins
 	@${MKDIR} ${WRKSRC}/MoCha
 	@${MV} ${WRKDIR}/*.R ${WRKSRC}/MoCha
 
 pre-configure:
 	@${REINPLACE_CMD} -e 's|@PORTVERSION@|${PORTVERSION}|g' \
 		${WRKSRC}/configure.ac
 
 # One .so for each .c in the mocha distfile
 do-install:
 	${MKDIR} ${STAGEDIR}${PREFIX}/libexec/bcftools
 	${INSTALL_PROGRAM} ${WRKSRC}/plugins/extendFMT.so \
 		${STAGEDIR}${PREFIX}/libexec/bcftools
 	${INSTALL_PROGRAM} ${WRKSRC}/plugins/mocha.so \
 		${STAGEDIR}${PREFIX}/libexec/bcftools
 	${INSTALL_PROGRAM} ${WRKSRC}/plugins/mochatools.so \
 		${STAGEDIR}${PREFIX}/libexec/bcftools
 	(cd ${WRKSRC}/MoCha && ${COPYTREE_SHARE} . ${STAGEDIR}${DATADIR})
 
 .include <bsd.port.mk>
diff --git a/biology/bio-mocha/distinfo b/biology/bio-mocha/distinfo
index 6485cffc5d72..9d05039e80b1 100644
--- a/biology/bio-mocha/distinfo
+++ b/biology/bio-mocha/distinfo
@@ -1,5 +1,5 @@
-TIMESTAMP = 1750428898
-SHA256 (bio-mocha_1.20-20240505.tar.gz) = 3beeb7f046b491fed162dfedd83a29b3eebb165fb1f2a25f44df7fa2f063793b
-SIZE (bio-mocha_1.20-20240505.tar.gz) = 66752
-SHA256 (samtools-bcftools-1.20-1.22_GH0.tar.gz) = 1608e547daf7bd5ab7d52dea57019f57da3fdfb29bc26dece1e8d01a999a9a2d
-SIZE (samtools-bcftools-1.20-1.22_GH0.tar.gz) = 3751342
+TIMESTAMP = 1787335796
+SHA256 (bio-mocha_1.22-20250819.tar.gz) = c5d4da549e710377335b17d5222c1253152b4b4b17b5b1dfef558debe3618c8e
+SIZE (bio-mocha_1.22-20250819.tar.gz) = 67361
+SHA256 (samtools-bcftools-1.22-1.24_GH0.tar.gz) = 386ac0d2fa51da312d01aada488ec98cf44ea4bac00d2a6c6d4993291b2f8e8c
+SIZE (samtools-bcftools-1.22-1.24_GH0.tar.gz) = 3800098