diff --git a/biology/py-pyrodigal/Makefile b/biology/py-pyrodigal/Makefile
index 67eb9672b9b0..7d135a8b0e3a 100644
--- a/biology/py-pyrodigal/Makefile
+++ b/biology/py-pyrodigal/Makefile
@@ -1,31 +1,31 @@
 PORTNAME=	pyrodigal
-DISTVERSION=	3.4.0
+DISTVERSION=	3.4.1
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Python binding for Prodigal, an ORF finder for genomes and metagenomes
-WWW=		https://github.com/althonos/pyrodigal
+WWW=		https://github.com/althonos/pyrodigal/
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/COPYING
 
 BUILD_DEPENDS=	${PYTHON_PKGNAMEPREFIX}cython3>0:lang/cython3@${PY_FLAVOR}
 TEST_DEPENDS=	${PYTHON_PKGNAMEPREFIX}archspec>0:devel/py-archspec@${PY_FLAVOR}
 
 USES=		python
 USE_PYTHON=	distutils concurrent autoplist pytest # cython: cython3 isn't yet supported by python.mk
 
 TEST_ENV=	${MAKE_ENV} PYTHONPATH=${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}
 
 post-build:
 	@cd ${BUILD_WRKSRC} && ${SETENV} ${MAKE_ENV} ${PYTHON_CMD} setup.py build_ext --inplace
 
 post-install:
 	@${STRIP_CMD} ${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}/pyrodigal/lib${PYTHON_EXT_SUFFIX}.so
 
 do-test:
 	@cd ${TEST_WRKSRC} && ${SETENV} ${TEST_ENV} ${PYTHON_CMD} -m unittest discover -vv
 
 .include <bsd.port.mk>
diff --git a/biology/py-pyrodigal/distinfo b/biology/py-pyrodigal/distinfo
index d3d07b0b3354..ce200fb3c956 100644
--- a/biology/py-pyrodigal/distinfo
+++ b/biology/py-pyrodigal/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1716179060
-SHA256 (pyrodigal-3.4.0.tar.gz) = d4714e8f9208b7768f89bca9f47013e9f18f978e4baf43176a67af9cb26c7c79
-SIZE (pyrodigal-3.4.0.tar.gz) = 2577063
+TIMESTAMP = 1716658828
+SHA256 (pyrodigal-3.4.1.tar.gz) = 65963ad8cf651f408758e7fa74cbf793eae9720310f0ef8781ee1a285d435326
+SIZE (pyrodigal-3.4.1.tar.gz) = 2577599
diff --git a/biology/py-pyrodigal/pkg-descr b/biology/py-pyrodigal/pkg-descr
index c56d5dda28d0..f33eb8de1a50 100644
--- a/biology/py-pyrodigal/pkg-descr
+++ b/biology/py-pyrodigal/pkg-descr
@@ -1,18 +1,18 @@
 Pyrodigal is a Python module that provides bindings to Prodigal using Cython.
 
 Features:
 The library now features everything from the original Prodigal CLI:
-* run mode selection: Choose between single mode, using a training sequence to
-  count nucleotide hexamers, or metagenomic mode, using pre-trained data from
-  different organisms (prodigal -p).
-* region masking: Prevent genes from being predicted across regions containing
-  unknown nucleotides (prodigal -m).
-* closed ends: Genes will be identified as running over edges if they are larger
-  than a certain size, but this can be disabled (prodigal -c).
+* run mode selection: Choose between single mode, using a training sequence
+  to count nucleotide hexamers, or metagenomic mode, using pre-trained data
+  from different organisms (prodigal -p).
+* region masking: Prevent genes from being predicted across regions
+  containing unknown nucleotides (prodigal -m).
+* closed ends: Genes will be identified as running over edges if they are
+  larger than a certain size, but this can be disabled (prodigal -c).
 * training configuration: During the training process, a custom translation
-  table can be given (prodigal -g), and the Shine-Dalgarno motif search can be
-  forcefully bypassed (prodigal -n)
-* output files: Output files can be written in a format mostly compatible with
-  the Prodigal binary, including the protein translations in FASTA format
-  (prodigal -a), the gene sequences in FASTA format (prodigal -d), or the
-  potential gene scores in tabular format (prodigal -s).
+  table can be given (prodigal -g), and the Shine-Dalgarno motif search can
+  be forcefully bypassed (prodigal -n)
+* output files: Output files can be written in a format mostly compatible
+  with the Prodigal binary, including the protein translations in FASTA
+  format (prodigal -a), the gene sequences in FASTA format (prodigal -d),
+  or the potential gene scores in tabular format (prodigal -s).