diff --git a/biology/bcftools/Makefile b/biology/bcftools/Makefile
index 11a2a1f9a31e..b810f468bf09 100644
--- a/biology/bcftools/Makefile
+++ b/biology/bcftools/Makefile
@@ -1,64 +1,64 @@
 PORTNAME=	bcftools
-PORTVERSION=	1.23.1
+PORTVERSION=	1.24
 CATEGORIES=	biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Tools for manipulating next-generation sequencing data
 WWW=		https://www.htslib.org/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
-BUILD_DEPENDS=	htslib>=1.22:biology/htslib
+BUILD_DEPENDS=	htslib>=1.24:biology/htslib
 LIB_DEPENDS=	libhts.so:biology/htslib
 TEST_DEPENDS=	bash:shells/bash
 RUN_DEPENDS=	bash:shells/bash
 
 USES=		autoreconf gmake localbase perl5 python:env shebangfix
 
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	samtools
 GNU_CONFIGURE=	yes
 GNU_CONFIGURE_MANPREFIX=	${PREFIX}/share
 SHEBANG_FILES=	misc/* test/test.pl
 
 OPTIONS_DEFINE=		GPL PERL
 GPL_DESC=		Enable modules that rely on GPL code
 PERL_DESC=		Enable external PERL scripts for advanced filtering
 OPTIONS_DEFAULT=	GPL PERL
 
 GPL_LIB_DEPENDS=	libgsl.so:math/gsl
 GPL_CONFIGURE_ENABLE=	libgsl
 PERL_USES=		perl5
 PERL_CONFIGURE_ENABLE=	perl-filters
 
 .include <bsd.port.options.mk>
 
 .if ${PORT_OPTIONS:MGPL}
 MAKE_ENV+=	USE_GPL=1
 LICENSE+=	GPLv3
 LICENSE_COMB=	multi
 .endif
 
 TEST_TARGET=	test
 
 pre-configure:
 	@${REINPLACE_CMD} -e 's|@PORTVERSION@|${PORTVERSION}|g' \
 		${WRKSRC}/configure.ac
 
 post-install:
 	${MV} ${STAGEDIR}${PREFIX}/bin/color-chrs.pl \
 		${STAGEDIR}${PREFIX}/bin/color-chrs
 	${MV} ${STAGEDIR}${PREFIX}/bin/guess-ploidy.py\
 	       ${STAGEDIR}${PREFIX}/bin/guess-ploidy
 	${MV} /${STAGEDIR}${PREFIX}/bin/plot-roh.py \
 		${STAGEDIR}${PREFIX}/bin/plot-roh
 	${MV} ${STAGEDIR}${PREFIX}/bin/run-roh.pl \
 		${STAGEDIR}${PREFIX}/bin/run-roh
 	${MV} ${STAGEDIR}${PREFIX}/bin/vcfutils.pl \
 		${STAGEDIR}${PREFIX}/bin/vcfutils
 	${MV} ${STAGEDIR}${PREFIX}/bin/plot-vcfstats \
 		${STAGEDIR}${PREFIX}/bin/
 
 .include <bsd.port.mk>
diff --git a/biology/bcftools/distinfo b/biology/bcftools/distinfo
index c92df2732f05..01572b40999c 100644
--- a/biology/bcftools/distinfo
+++ b/biology/bcftools/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1774783023
-SHA256 (samtools-bcftools-1.23.1_GH0.tar.gz) = e3b47ff5f771389ba754b12428873642db15ee2baf06cd3ac1a214a39a74ec58
-SIZE (samtools-bcftools-1.23.1_GH0.tar.gz) = 3764835
+TIMESTAMP = 1783682254
+SHA256 (samtools-bcftools-1.24_GH0.tar.gz) = 386ac0d2fa51da312d01aada488ec98cf44ea4bac00d2a6c6d4993291b2f8e8c
+SIZE (samtools-bcftools-1.24_GH0.tar.gz) = 3800098
diff --git a/biology/bcftools/pkg-plist b/biology/bcftools/pkg-plist
index 2edeeaca650c..bbdd6ce65f41 100644
--- a/biology/bcftools/pkg-plist
+++ b/biology/bcftools/pkg-plist
@@ -1,53 +1,53 @@
 bin/bcftools
 bin/color-chrs
 bin/gff2gff
 bin/gff2gff.py
 bin/guess-ploidy
 bin/plot-roh
 bin/plot-vcfstats
 bin/roh-viz
 bin/run-roh
 bin/vcfutils
 bin/vrfs-variances
 libexec/bcftools/GTisec.so
 libexec/bcftools/GTsubset.so
 libexec/bcftools/ad-bias.so
 libexec/bcftools/add-variantkey.so
 libexec/bcftools/af-dist.so
 libexec/bcftools/allele-length.so
 libexec/bcftools/check-ploidy.so
 libexec/bcftools/check-sparsity.so
 libexec/bcftools/color-chrs.so
 libexec/bcftools/contrast.so
 libexec/bcftools/counts.so
 libexec/bcftools/dosage.so
 libexec/bcftools/fill-AN-AC.so
 libexec/bcftools/fill-from-fasta.so
 libexec/bcftools/fill-tags.so
 libexec/bcftools/fixploidy.so
 libexec/bcftools/fixref.so
 libexec/bcftools/frameshifts.so
 libexec/bcftools/guess-ploidy.so
 libexec/bcftools/gvcfz.so
 libexec/bcftools/impute-info.so
 libexec/bcftools/indel-stats.so
 libexec/bcftools/isecGT.so
 libexec/bcftools/mendelian2.so
 libexec/bcftools/missing2ref.so
 libexec/bcftools/parental-origin.so
 libexec/bcftools/prune.so
 libexec/bcftools/remove-overlaps.so
 libexec/bcftools/scatter.so
 libexec/bcftools/setGT.so
 libexec/bcftools/smpl-stats.so
 libexec/bcftools/split-vep.so
 libexec/bcftools/split.so
 libexec/bcftools/tag2tag.so
-libexec/bcftools/trio-dnm2.so
+libexec/bcftools/trio-dnm3.so
 libexec/bcftools/trio-stats.so
 libexec/bcftools/trio-switch-rate.so
 libexec/bcftools/variant-distance.so
 libexec/bcftools/variantkey-hex.so
 libexec/bcftools/vcf2table.so
 libexec/bcftools/vrfs.so
 share/man/man1/bcftools.1.gz