diff --git a/biology/vsearch/Makefile b/biology/vsearch/Makefile
index 9aeeeb61cb30..89da4b6fea16 100644
--- a/biology/vsearch/Makefile
+++ b/biology/vsearch/Makefile
@@ -1,29 +1,29 @@
 PORTNAME=		vsearch
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.30.6
+DISTVERSION=		2.31.0
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Versatile open-source tool for metagenomics
 WWW=		https://github.com/torognes/vsearch
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64le
 ONLY_FOR_ARCHS_REASON=	Explicitly enumerated by upstream developers
 
 USES=		autoreconf ghostscript:build gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	torognes
 GNU_CONFIGURE=	yes
 GNU_CONFIGURE_MANPREFIX=	${PREFIX}/share
 
 PLIST_FILES=	bin/vsearch share/man/man1/vsearch.1.gz
 PORTDOCS=	*
 
 OPTIONS_DEFINE=	DOCS
 OPTIONS_SUB=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/vsearch/distinfo b/biology/vsearch/distinfo
index cb4fcea1d345..e084e4c70095 100644
--- a/biology/vsearch/distinfo
+++ b/biology/vsearch/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1774696782
-SHA256 (torognes-vsearch-v2.30.6_GH0.tar.gz) = 6aa4d7f5ad07774d8e3477bc9e17a91d182f5b7f92c04e43d1bc09d539086d34
-SIZE (torognes-vsearch-v2.30.6_GH0.tar.gz) = 338240
+TIMESTAMP = 1777740585
+SHA256 (torognes-vsearch-v2.31.0_GH0.tar.gz) = fb6348fe38107cdcde6e3651913aa54eb8233fcd3379ab9e7fc936c090e05915
+SIZE (torognes-vsearch-v2.31.0_GH0.tar.gz) = 609494