diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index 4ae1f9786100..8f934f4da440 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,34 +1,33 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.11.0
-PORTREVISION=	5
+DISTVERSION=	2.13.0
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Cross-platform and ultrafast toolkit for FASTA/Q file manipulation
 WWW=		https://bioinf.shenwei.me/seqkit \
 		https://github.com/shenwei356/seqkit
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 BROKEN_i386=	compilation fails: vendor/github.com/ulikunitz/xz/lzma/reader.go:33:15: cannot use 1 << 31 (untyped int constant 2147483648) as int value in assignment (overflows)
 
 USES=		go:1.24+,modules
 USE_GITHUB=	nodefault
 GH_TUPLE=	golang:sys:v0.6.0:sys
 
 GO_MODULE=	github.com/shenwei356/seqkit/v2
 GO_TARGET=	./${PORTNAME}
 
 PLIST_FILES=	bin/${PORTNAME}
 
 pre-patch:
 	${RM} -r ${WRKSRC}/vendor/golang.org/x/sys
 	${LN} -s ${WRKDIR}/sys-* ${WRKSRC}/vendor/golang.org/x/sys
 
 do-test: # from https://bioinf.shenwei.me/seqkit/benchmark/
 	@cd ${WRKSRC}/tests && \
 		${GO_WRKDIR_BIN}/seqkit stat *.fa
 
 .include <bsd.port.mk>
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 065b2631ac71..78a8c39207b3 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,7 +1,7 @@
-TIMESTAMP = 1764090448
-SHA256 (go/biology_seqkit/seqkit-v2.11.0/v2.11.0.mod) = 6dcc9f5952dbc480064586db883831cf6f0461139b46b5273e9b63515b2b6695
-SIZE (go/biology_seqkit/seqkit-v2.11.0/v2.11.0.mod) = 2572
-SHA256 (go/biology_seqkit/seqkit-v2.11.0/v2.11.0.zip) = 46f19ed0fe981caad8c0cf94222517a589d644ab2d9c51dcabaf81f6204e8651
-SIZE (go/biology_seqkit/seqkit-v2.11.0/v2.11.0.zip) = 34100274
-SHA256 (go/biology_seqkit/seqkit-v2.11.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
-SIZE (go/biology_seqkit/seqkit-v2.11.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234
+TIMESTAMP = 1775935878
+SHA256 (go/biology_seqkit/seqkit-v2.13.0/v2.13.0.mod) = 666e8a92ef4d1692c2d84ce1e695ae638b42cc28b6c163a50a973b1b16cb7fde
+SIZE (go/biology_seqkit/seqkit-v2.13.0/v2.13.0.mod) = 2607
+SHA256 (go/biology_seqkit/seqkit-v2.13.0/v2.13.0.zip) = c2c1c46e687baaf48c866f5c10119708e53e384843f299748f5de1293cc3b58e
+SIZE (go/biology_seqkit/seqkit-v2.13.0/v2.13.0.zip) = 34111397
+SHA256 (go/biology_seqkit/seqkit-v2.13.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
+SIZE (go/biology_seqkit/seqkit-v2.13.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234