diff --git a/biology/vsearch/Makefile b/biology/vsearch/Makefile
index c42444db8a21..6d6ded03e517 100644
--- a/biology/vsearch/Makefile
+++ b/biology/vsearch/Makefile
@@ -1,29 +1,29 @@
 PORTNAME=		vsearch
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.27.1
+DISTVERSION=		2.28.1
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Versatile open-source tool for metagenomics
 WWW=		https://github.com/torognes/vsearch
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
 ONLY_FOR_ARCHS=		aarch64 amd64 powerpc64le
 ONLY_FOR_ARCHS_REASON=	Explicitly enumerated by upstream developers
 
 USES=		autoreconf ghostscript:build gmake localbase
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	torognes
 GNU_CONFIGURE=	yes
 GNU_CONFIGURE_MANPREFIX=	${PREFIX}/share
 
 PLIST_FILES=	bin/vsearch share/man/man1/vsearch.1.gz
 PORTDOCS=	*
 
 OPTIONS_DEFINE=	DOCS
 OPTIONS_SUB=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/vsearch/distinfo b/biology/vsearch/distinfo
index 7eea2ede671c..f746cec601da 100644
--- a/biology/vsearch/distinfo
+++ b/biology/vsearch/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1712512768
-SHA256 (torognes-vsearch-v2.27.1_GH0.tar.gz) = 54666bcd9434fb7636c5b292a67e7c194e809e631bbe8f4f86b85fb47e863abc
-SIZE (torognes-vsearch-v2.27.1_GH0.tar.gz) = 269179
+TIMESTAMP = 1714566699
+SHA256 (torognes-vsearch-v2.28.1_GH0.tar.gz) = 4f8bf0ad43fef77e573d152b59f55a1f81eb84c22d6545911757e6108f8de21c
+SIZE (torognes-vsearch-v2.28.1_GH0.tar.gz) = 272918