diff --git a/biology/diamond/Makefile b/biology/diamond/Makefile
index 244ed8c79bdf..5e85f2a15b62 100644
--- a/biology/diamond/Makefile
+++ b/biology/diamond/Makefile
@@ -1,24 +1,24 @@
 PORTNAME=	diamond
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.2.2
+DISTVERSION=	2.2.3
 CATEGORIES=	biology
 
 MAINTAINER=	jrm@FreeBSD.org
 COMMENT=	BLAST-compatible local sequence aligner
 WWW=		http://ab.inf.uni-tuebingen.de/software/diamond/ \
 		https://github.com/bbuchfink/diamond
 
 LICENSE=	GPLv3
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 ONLY_FOR_ARCHS=	aarch64 amd64 powerpc64 powerpc64le
 
 USES=		cmake localbase sqlite
 USE_GITHUB=	yes
 GH_ACCOUNT=	bbuchfink
 
 TEST_TARGET=	test
 
 PLIST_FILES=	bin/diamond
 
 .include <bsd.port.mk>
diff --git a/biology/diamond/distinfo b/biology/diamond/distinfo
index b6253b1ba42d..a2988225ab43 100644
--- a/biology/diamond/distinfo
+++ b/biology/diamond/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1782163658
-SHA256 (bbuchfink-diamond-v2.2.2_GH0.tar.gz) = 35cb389dad7326515267b21a04256382853754d32b48eae6fd8a56fbb191e1c6
-SIZE (bbuchfink-diamond-v2.2.2_GH0.tar.gz) = 14394953
+TIMESTAMP = 1785209889
+SHA256 (bbuchfink-diamond-v2.2.3_GH0.tar.gz) = 6199f96b294adcc2f4fde72deaa5757cb5b4ab4657c0d84c866047a70031742e
+SIZE (bbuchfink-diamond-v2.2.3_GH0.tar.gz) = 15053281
diff --git a/biology/diamond/files/patch-src_util_io_file.cpp b/biology/diamond/files/patch-src_util_io_file.cpp
new file mode 100644
index 000000000000..110aff8b7e35
--- /dev/null
+++ b/biology/diamond/files/patch-src_util_io_file.cpp
@@ -0,0 +1,11 @@
+--- src/util/io/file.cpp.orig	2026-07-13 11:36:09 UTC
++++ src/util/io/file.cpp
+@@ -21,6 +21,8 @@ along with this program.  If not, see <http://www.gnu.
+ #ifdef _WIN32
+ #include <io.h>
+ #include <fcntl.h>
++#else
++#include <unistd.h> // unlink
+ #endif
+ #include <string.h> // strerror
+ #include "file.h"