diff --git a/biology/hyphy/Makefile b/biology/hyphy/Makefile
index c0d6bd4541ba..1a44d2bb0695 100644
--- a/biology/hyphy/Makefile
+++ b/biology/hyphy/Makefile
@@ -1,28 +1,28 @@
 PORTNAME=	hyphy
-DISTVERSION=	2.5.42
+DISTVERSION=	2.5.43
 CATEGORIES=	biology
 
 MAINTAINER=	jrm@FreeBSD.org
 COMMENT=	Hypothesis testing using Phylogenies
 WWW=		https://www.hyphy.org/
 
 LICENSE=	MIT
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 ONLY_FOR_ARCHS=	aarch64 amd64 armv7 i386 powerpc powerpc64 powerpc64le
 
 LIB_DEPENDS=	libcurl.so:ftp/curl
 
 USES=		cmake:insource,testing localbase:ldflags # tests fail in outsource build, see https://github.com/veg/hyphy/issues/1528
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	veg
 
 OPTIONS_DEFINE=	AVX
 AVX_DESC=	Advanced vector extensions support
 
 AVX_CMAKE_OFF=	-DNOAVX:BOOL=ON
 
 # 2 tests fail, see https://github.com/veg/hyphy/issues/1529
 
 .include <bsd.port.mk>
diff --git a/biology/hyphy/distinfo b/biology/hyphy/distinfo
index 668938dee708..11533428b33d 100644
--- a/biology/hyphy/distinfo
+++ b/biology/hyphy/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1663619549
-SHA256 (veg-hyphy-2.5.42_GH0.tar.gz) = 7b83694fdc42f9c1ae39b1b80f1c614e6f23e2997a32c147494eadc242a81617
-SIZE (veg-hyphy-2.5.42_GH0.tar.gz) = 5634392
+TIMESTAMP = 1672008666
+SHA256 (veg-hyphy-2.5.43_GH0.tar.gz) = 9328916267811a95dbbff94b51066dfc481f8d49ae0cf4d58e8384c58afbea50
+SIZE (veg-hyphy-2.5.43_GH0.tar.gz) = 5634198