diff --git a/biology/py-gtfparse/Makefile b/biology/py-gtfparse/Makefile
index 114fb6017c25..b2bb5e43548a 100644
--- a/biology/py-gtfparse/Makefile
+++ b/biology/py-gtfparse/Makefile
@@ -1,22 +1,23 @@
 PORTNAME=	gtfparse
-DISTVERSION=	1.3.0
-PORTREVISION=	1
+DISTVERSION=	2.5.0
 CATEGORIES=	biology python
-MASTER_SITES=	PYPI
+#MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Parsing tools for GTF (gene transfer format) files
 WWW=		https://github.com/openvax/gtfparse
 
 LICENSE=	APACHE20
 
-RUN_DEPENDS=	${PYNUMPY} \
-		${PYTHON_PKGNAMEPREFIX}pandas>=0.15:math/py-pandas@${PY_FLAVOR}
+BUILD_DEPENDS=	${PY_SETUPTOOLS}
+RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}pandas>0:math/py-pandas@${PY_FLAVOR} \
+		${PYTHON_PKGNAMEPREFIX}polars>=0.20.2:misc/py-polars@${PY_FLAVOR} \
+		${PYTHON_PKGNAMEPREFIX}pyarrow>=14.0.2:databases/py-pyarrow@${PY_FLAVOR}
 
 USES=		python
-USE_PYTHON=	distutils autoplist
+USE_PYTHON=	pep517 autoplist pytest # tests from the PYPI tarball fail because 'data' module is missing, and the GH repository doesn't have tags
 
 NO_ARCH=	yes
 
 .include <bsd.port.mk>
diff --git a/biology/py-gtfparse/distinfo b/biology/py-gtfparse/distinfo
index c475bd4a83f1..cf2b7fe4fe20 100644
--- a/biology/py-gtfparse/distinfo
+++ b/biology/py-gtfparse/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1678604725
-SHA256 (gtfparse-1.3.0.tar.gz) = d957f18e5f70413f89a28ef83068c461b6407eb38fd30e99b8da3d69143527b1
-SIZE (gtfparse-1.3.0.tar.gz) = 16740
+TIMESTAMP = 1731429071
+SHA256 (gtfparse-2.5.0.tar.gz) = 9fea54811cd87f597a110a49dc1b1b6a3325ffb7d1f36ecc62c32d14d3eb9493
+SIZE (gtfparse-2.5.0.tar.gz) = 17228