diff --git a/biology/py-ont-fast5-api/Makefile b/biology/py-ont-fast5-api/Makefile
index 297898586ca5..adfb3dc5fdca 100644
--- a/biology/py-ont-fast5-api/Makefile
+++ b/biology/py-ont-fast5-api/Makefile
@@ -1,44 +1,44 @@
 PORTNAME=	ont-fast5-api
 DISTVERSIONPREFIX=	release_
-DISTVERSION=	4.0.2
-PORTREVISION=	4
+DISTVERSION=	4.1.3
 CATEGORIES=	biology python
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	jwb@FreeBSD.org
 COMMENT=	Interface to HDF5 files in Oxford Nanopore .fast5 format
-WWW=		https://github.com/nanoporetech/ont_fast5_api/
+WWW=		https://github.com/nanoporetech/ont_fast5_api
 
 LICENSE=	MPL20
 LICENSE_FILE=	${WRKSRC}/LICENSE.md
 
 LIB_DEPENDS=	libvbz_hdf_plugin.so:archivers/vbz-compression
-RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}numpy>=1.16:math/py-numpy@${PY_FLAVOR} \
-		${PYTHON_PKGNAMEPREFIX}h5py>=2.2.0:science/py-h5py@${PY_FLAVOR} \
-		${PYTHON_PKGNAMEPREFIX}six>0:devel/py-six@${PY_FLAVOR} \
-		${PYTHON_PKGNAMEPREFIX}progressbar>0:misc/py-progressbar@${PY_FLAVOR}
+RUN_DEPENDS=	${PYNUMPY} \
+		${PYTHON_PKGNAMEPREFIX}h5py>=3:science/py-h5py@${PY_FLAVOR} \
+		${PYTHON_PKGNAMEPREFIX}packaging>0:devel/py-packaging@${PY_FLAVOR} \
+		${PYTHON_PKGNAMEPREFIX}progressbar>=2.3.1:misc/py-progressbar@${PY_FLAVOR}
 TEST_DEPENDS=	${PYTHON_PKGNAMEPREFIX}pip>=0:devel/py-pip@${PY_FLAVOR}
 
 USES=		python
+# Does not yet build with pep517
 USE_PYTHON=	autoplist concurrent distutils
 USE_GITHUB=	yes
 
 GH_ACCOUNT=	nanoporetech
 GH_PROJECT=	ont_fast5_api
 
 NO_ARCH=	yes
 NO_BUILD=	yes
 
 post-patch:
 	${RM} ${WRKSRC}/ont_fast5_api/vbz_plugin/*
 
 pre-configure:
 	@${REINPLACE_CMD} -e 's|progressbar33|progressbar|g' \
 		${WRKSRC}/setup.py \
 		${WRKSRC}/README.rst \
 		${WRKSRC}/ont_fast5_api/conversion_tools/conversion_utils.py
 
 do-test:
 	@cd ${WRKSRC} && ${PYTHON_CMD} ${PYDISTUTILS_SETUP} test
 
 .include <bsd.port.mk>
diff --git a/biology/py-ont-fast5-api/distinfo b/biology/py-ont-fast5-api/distinfo
index d6298ee5ba57..279215939d9a 100644
--- a/biology/py-ont-fast5-api/distinfo
+++ b/biology/py-ont-fast5-api/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1648128656
-SHA256 (nanoporetech-ont_fast5_api-release_4.0.2_GH0.tar.gz) = c078dc0d20b02cd9807105415f259e099f1adc8b922d67a1a9fe04321c098361
-SIZE (nanoporetech-ont_fast5_api-release_4.0.2_GH0.tar.gz) = 4082466
+TIMESTAMP = 1788736924
+SHA256 (nanoporetech-ont_fast5_api-release_4.1.3_GH0.tar.gz) = 80e021a6fbb1c442d0d782ca36815e4cc653bd352431489995285e96fb98d96c
+SIZE (nanoporetech-ont_fast5_api-release_4.1.3_GH0.tar.gz) = 4443485