diff --git a/devel/nanobind/Makefile b/devel/nanobind/Makefile
index c8ee7f1ff77b..4156b6684695 100644
--- a/devel/nanobind/Makefile
+++ b/devel/nanobind/Makefile
@@ -1,71 +1,71 @@
 PORTNAME=	nanobind
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.13.0
+DISTVERSION=	3.0.1
 CATEGORIES=	devel
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	Tiny and efficient C++/Python bindings (C++ version)
 WWW=		https://nanobind.readthedocs.io/en/latest/ \
 		https://github.com/wjakob/nanobind
 
 LICENSE=	BSD3CLAUSE
 LICENSE_FILE=	${WRKSRC}/LICENSE
 
 BUILD_DEPENDS=	robin-map>0:devel/robin-map
 RUN_DEPENDS=	robin-map>0:devel/robin-map
 TEST_DEPENDS=	eigen>0:math/eigen3 \
 		gmake:devel/gmake \
 		pytest:devel/py-pytest@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}jax>0:math/py-jax@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}numpy>0:math/py-numpy@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}scipy>0:science/py-scipy@${PY_FLAVOR} \
 		${PYTHON_PKGNAMEPREFIX}typing-extensions>0:devel/py-typing-extensions@${PY_FLAVOR}
 #		${PYTHON_PKGNAMEPREFIX}tensorflow>0:science/py-tensorflow@${PY_FLAVORA} # tensorflow tests crash the python process
 
 USES=		cmake:testing localbase python:test
 
 USE_GITHUB=	yes
 GH_ACCOUNT=	wjakob
 
 CMAKE_ARGS=	-DNB_INSTALL_DATADIR=${PREFIX}
 CMAKE_ON=	NB_CREATE_INSTALL_RULES
 CMAKE_OFF=	NB_USE_SUBMODULE_DEPS \
 		NB_TEST_FREE_THREADED \
 		NB_TEST \
 		CMAKE_SKIP_INSTALL_RULES
 CMAKE_TESTING_ON=	NB_TEST
 CMAKE_TESTING_TARGET=	copy-tests
 CXXFLAGS+=	-I${WRKSRC}/include \
 		-I${PYTHON_INCLUDEDIR}
 
 INSTALL_TARGET=	install
 
 TEST_WRKSRC=	${BUILD_WRKSRC}/tests
 
 NO_ARCH=	yes
 
 post-install:
 	# workaround for https://github.com/wjakob/nanobind/issues/1300
 	@${RM} \
 		${STAGEDIR}${PREFIX}/cmake/darwin-ld-cpython.sym \
 		${STAGEDIR}${PREFIX}/cmake/darwin-ld-pypy.sym \
 		${STAGEDIR}${PREFIX}/cmake/darwin-python-path.py
 	# correct path to the src directory in cmake file
 	@${REINPLACE_CMD} -i '' -e 's|$${NB_DIR}/src/|${DATADIR}/src/|g' \
 		${STAGEDIR}${PREFIX}/cmake/nanobind-config.cmake
 
 post-test:
 	@cd ${TEST_WRKSRC} && \
 		pytest .
 
-# tests as of 2.12.0:
-# Results (41.59s):
-#          2 failed
-#       1486 passed
-#         19 skipped
-#          2 warnings
+# tests as of 3.0.1:
+# Results (46.19s):
+#      610 passed
+#        1 failed
+#          - test_inter_module.py:26 test02_reload_module
+#       92 skipped
 
 # cmake installs files into non-standard locations, see https://github.com/wjakob/nanobind/issues/810
 # hostile upstream, do not create bug reports
 
 .include <bsd.port.mk>
diff --git a/devel/nanobind/distinfo b/devel/nanobind/distinfo
index 3bb0b5dde8b6..55ac7d5a6a65 100644
--- a/devel/nanobind/distinfo
+++ b/devel/nanobind/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1781768189
-SHA256 (wjakob-nanobind-v2.13.0_GH0.tar.gz) = cb25a582ccade4b6067bc73c78b84ad9dbd0bbe0e537320711d18015ccafc4ef
-SIZE (wjakob-nanobind-v2.13.0_GH0.tar.gz) = 989569
+TIMESTAMP = 1789104380
+SHA256 (wjakob-nanobind-v3.0.1_GH0.tar.gz) = 34ded7cf2292f08a92c45490a095e76334a57751bbae03a8c83241803bf19623
+SIZE (wjakob-nanobind-v3.0.1_GH0.tar.gz) = 1080674
diff --git a/devel/nanobind/files/patch-cmake_nanobind-config.cmake b/devel/nanobind/files/patch-cmake_nanobind-config.cmake
new file mode 100644
index 000000000000..2b1d48319810
--- /dev/null
+++ b/devel/nanobind/files/patch-cmake_nanobind-config.cmake
@@ -0,0 +1,20 @@
+-- remove -mtls-dialect=gnu2 since it breaks generated binaries
+-- and they can't be loaded with unknown relocation entry types
+
+--- cmake/nanobind-config.cmake.orig	2026-09-11 05:35:01 UTC
++++ cmake/nanobind-config.cmake
+@@ -108,10 +108,10 @@ set(NB_OPT_SIZE $<OR:$<CONFIG:Release>,$<CONFIG:MinSiz
+ # Probe for the faster TLSDESC thread-local storage ABI (Linux/x86_64)
+ # ---------------------------------------------------------------------------
+ 
+-if (NOT (MSVC OR WIN32 OR APPLE))
+-  include(CheckCXXCompilerFlag)
+-  check_cxx_compiler_flag(-mtls-dialect=gnu2 NB_HAS_MTLS_GNU2)
+-endif()
++#if (NOT (MSVC OR WIN32 OR APPLE))
++#  include(CheckCXXCompilerFlag)
++#  check_cxx_compiler_flag(-mtls-dialect=gnu2 NB_HAS_MTLS_GNU2)
++#endif()
+ 
+ # ---------------------------------------------------------------------------
+ # Helper function to handle undefined CPython API symbols on macOS
diff --git a/devel/nanobind/pkg-plist b/devel/nanobind/pkg-plist
index 5405408c622b..d176d9bef349 100644
--- a/devel/nanobind/pkg-plist
+++ b/devel/nanobind/pkg-plist
@@ -1,80 +1,81 @@
 cmake/nanobind-config-version.cmake
 cmake/nanobind-config.cmake
 include/nanobind/eigen/dense.h
 include/nanobind/eigen/sparse.h
 include/nanobind/eigen/tensor.h
 include/nanobind/eval.h
 include/nanobind/intrusive/counter.h
 include/nanobind/intrusive/counter.inl
 include/nanobind/intrusive/ref.h
 include/nanobind/make_iterator.h
 include/nanobind/nanobind.h
 include/nanobind/nb_accessor.h
 include/nanobind/nb_attr.h
+include/nanobind/nb_backend.h
+include/nanobind/nb_backend_slots.h
 include/nanobind/nb_call.h
 include/nanobind/nb_cast.h
 include/nanobind/nb_class.h
 include/nanobind/nb_defs.h
 include/nanobind/nb_descr.h
-include/nanobind/nb_enums.h
 include/nanobind/nb_error.h
 include/nanobind/nb_func.h
-include/nanobind/nb_lib.h
 include/nanobind/nb_misc.h
+include/nanobind/nb_platform.h
 include/nanobind/nb_python.h
 include/nanobind/nb_traits.h
 include/nanobind/nb_tuple.h
 include/nanobind/nb_types.h
 include/nanobind/ndarray.h
 include/nanobind/operators.h
 include/nanobind/stl/array.h
 include/nanobind/stl/bind_map.h
 include/nanobind/stl/bind_vector.h
 include/nanobind/stl/chrono.h
 include/nanobind/stl/complex.h
-include/nanobind/stl/detail/chrono.h
 include/nanobind/stl/detail/nb_array.h
 include/nanobind/stl/detail/nb_dict.h
 include/nanobind/stl/detail/nb_list.h
 include/nanobind/stl/detail/nb_optional.h
 include/nanobind/stl/detail/nb_set.h
 include/nanobind/stl/detail/traits.h
 include/nanobind/stl/filesystem.h
 include/nanobind/stl/function.h
 include/nanobind/stl/list.h
 include/nanobind/stl/map.h
 include/nanobind/stl/optional.h
 include/nanobind/stl/pair.h
 include/nanobind/stl/set.h
 include/nanobind/stl/shared_ptr.h
 include/nanobind/stl/string.h
 include/nanobind/stl/string_view.h
 include/nanobind/stl/tuple.h
 include/nanobind/stl/unique_ptr.h
 include/nanobind/stl/unordered_map.h
 include/nanobind/stl/unordered_set.h
 include/nanobind/stl/variant.h
 include/nanobind/stl/vector.h
 include/nanobind/stl/wstring.h
 include/nanobind/trampoline.h
 include/nanobind/typing.h
 %%DATADIR%%/__init__.py
 %%DATADIR%%/__main__.py
 %%DATADIR%%/src/buffer.h
 %%DATADIR%%/src/common.cpp
 %%DATADIR%%/src/error.cpp
 %%DATADIR%%/src/hash.h
 %%DATADIR%%/src/implicit.cpp
-%%DATADIR%%/src/nb_abi.h
+%%DATADIR%%/src/nb_backend.cpp
 %%DATADIR%%/src/nb_combined.cpp
+%%DATADIR%%/src/nb_datetime.cpp
 %%DATADIR%%/src/nb_enum.cpp
 %%DATADIR%%/src/nb_ft.cpp
 %%DATADIR%%/src/nb_ft.h
 %%DATADIR%%/src/nb_func.cpp
 %%DATADIR%%/src/nb_internals.cpp
 %%DATADIR%%/src/nb_internals.h
 %%DATADIR%%/src/nb_ndarray.cpp
 %%DATADIR%%/src/nb_static_property.cpp
 %%DATADIR%%/src/nb_type.cpp
 %%DATADIR%%/src/trampoline.cpp
 %%DATADIR%%/stubgen.py