diff --git a/biology/py-mrcfile/Makefile b/biology/py-mrcfile/Makefile
index f82721d6a194..aaad982b84cd 100644
--- a/biology/py-mrcfile/Makefile
+++ b/biology/py-mrcfile/Makefile
@@ -1,22 +1,27 @@
 PORTNAME=	mrcfile
-DISTVERSION=	1.5.3
-PORTREVISION=	3
+DISTVERSION=	1.5.4
 CATEGORIES=	biology python
 MASTER_SITES=	PYPI
 PKGNAMEPREFIX=	${PYTHON_PKGNAMEPREFIX}
 
 MAINTAINER=	yuri@FreeBSD.org
 COMMENT=	MRC file I/O library which is used in structural biology
 WWW=		https://github.com/ccpem/mrcfile
 
 LICENSE=	BSD3CLAUSE
 LICENSE_FILE=	${WRKSRC}/LICENSE.txt
 
+BUILD_DEPENDS=	${PY_SETUPTOOLS} \
+		${PYTHON_PKGNAMEPREFIX}wheel>0:devel/py-wheel@${PY_FLAVOR}
 RUN_DEPENDS=	${PYTHON_PKGNAMEPREFIX}numpy>=1.16:math/py-numpy@${PY_FLAVOR}
 
 USES=		python
-USE_PYTHON=	distutils concurrent autoplist
+USE_PYTHON=	pep517 concurrent autoplist pytest
 
 NO_ARCH=	yes
 
+TEST_ENV=	${MAKE_ENV} PYTHONPATH=${STAGEDIR}${PYTHONPREFIX_SITELIBDIR}
+
+# tests as of 1.5.4: 860 passed, 7 failed
+
 .include <bsd.port.mk>
diff --git a/biology/py-mrcfile/distinfo b/biology/py-mrcfile/distinfo
index 61d111500cd6..3f0366fcbb49 100644
--- a/biology/py-mrcfile/distinfo
+++ b/biology/py-mrcfile/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1721972673
-SHA256 (mrcfile-1.5.3.tar.gz) = 3f304c02cb9f0900b26683679c5d3d750da64b5c370b58d69af8a8ddf720c0ce
-SIZE (mrcfile-1.5.3.tar.gz) = 57453
+TIMESTAMP = 1780912628
+SHA256 (mrcfile-1.5.4.tar.gz) = 8fa9eb2e237d87d99b301c61bf02854dd85a451db25eefcbe142c09b36d8d601
+SIZE (mrcfile-1.5.4.tar.gz) = 52052606